BioLegend 698905 PE anti-STAT3

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BioLegend 698905 PE anti-STAT3 Phospho (Ser727)
产品简介：
产品品牌：BioLegend
产品货号：698905
产品名称：BioLegend 698905 PE anti-STAT3 Phospho (Ser727)
产品规格：25 tests

产品说明：
Clone   A16089B (See other available formats)
Regulatory Status   RUO
Other Names
Signal transducer and activator of transcription 3, Acute-phase response factor (APRF), HIES, ADMIO
Isotype   Mouse IgG1, κ

描述
STAT3转录因子是许多细胞因子和生长因子受体的重要信号分子，是小鼠胎儿发育所必需的。STAT3是STAT（信号转导子和转录激活子）蛋白家族的88kD成员，其响应细胞因子受体相关激酶活性而被磷酸化。Stat3被Tyr705的磷酸化激活，从而诱导二聚化、核易位和DNA结合。据报道，转录激活通过MAPK或mTOR途径受Ser727磷酸化的调节。
STAT3形成同源和异源三聚体，并参与细胞生长和凋亡所需基因的激活。STAT3还参与gp130信号传导，并与各种急性期蛋白启动子中的IL-6反应元件结合。STAT3通过IFN、EGF、FGF、IL-5、HGF、LIF和BMP2的信号传导而磷酸化。STAT3活性被PIAS3和GRIM-19抑制，并且也可以被Rac1蛋白调节。
The STAT3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors and is required for murine fetal development. STAT3 is an 88 kD member of the STAT (signal transducer and activators of transcription) protein family that is phosphorylated in response to a cytokine receptor-associated kinase activity. Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding. Transcriptional activation was reported to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways.

STAT3 forms both homo- and heterotrimers and is involved in the activation of genes required for cell growth and apoptosis. STAT3 is also involved in gp130 signaling and binds to IL-6 response elements in various acute phase protein promoters. STAT3 is phosphorylated by signaling from IFNs, EGF, FGF, IL-5, HGF, LIF, and BMP2. STAT3 activity is inhibited by PIAS3 and GRIM-19 and can also be regulated by the Rac1 protein.

Product Details
Verified Reactivity   Human
Antibody Type   Monoclonal
Host Species   Mouse
Immunogen
Human Stat3 peptide phosphorylated at Ser 727. Complete Freund's adjuvant.

Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.

Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)

Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application   ICFC - Quality tested

Recommended Usage
Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Antigen Details
Structure
STAT3 is a 770 amino acid protein of 88 kD. It consists of a DNA binding domain, a SH2 domain, a regulatory tyrosine responsible for binding of SH2 domain, and a C-terminal transactivation domain.
Distribution   Ubiquitous

Function
STAT3 is also involved in gp130 signaling and binds to IL-6 response elements in various acute phase protein promoters. STAT3 is phosphorylated by signaling from IFNs, EGF, FGF, IL-5, HGF, LIF, and BMP2. STAT3 activity is inhibited by PIAS3 and GRIM-19 and can also be regulated by the Rac1 protein.

Interaction
When activated, STAT3 dimerizes and translocates to the nucleus where it regulates gene expression.

Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Transcription Factors
Molecular Family    Nuclear Markers, Phospho-Proteins

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