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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名:
POLYVIEW® PLUS AP (anti-rabbit) reagent
- 应用范围:
IHC
- 库存:
99
- 供应商:
欣博盛生物
- 保存条件:
+4°C
- 规格:
150 tests
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文献和实验Testing Rabbit Bleeds By Elisa
at 1/10 (50 ul/well). Remove primary antibody and rinse wells 4Xs with TBST. Incubate in secondary antibody (1/5000 Goat anti-rabbit conjugated to AP) diluted in Blocking Buffer for 1 hr at RT (50 ul/well). Remove secondary antibody
Immunostaining with Anti-Digoxigenin Alkaline Phosphatase
Materials Anti-digoxigenin-AP conjugate (Roche #11093274910, 150U/200 uL, $168) Levamisole (Sigma #T1512, 2 g, $10) (blocks endogenous phosphatase activity) BCIP (Roche #11383221001, 3 mL, $49) NBT (Roche #11383213001, 3 mL, $48)
Testing Rabbit Bleeds By Elisa
antibody diluted in Blocking Buffer for 2 hr at RT. I do tripling dilutions beginning at 1/10 (50 ul/well). Remove primary antibody and rinse wells 4Xs with TBST. Incubate in secondary antibody (1/5000 Goat anti-rabbit conjugated to AP
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