- ￥13293
- Mabtech
- 3430-6-1000
- 瑞典
- 2025年07月15日
- ELISA,ELISpot等
- human
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
anti-human IL-10 mAb 12G8, biotinylated
- 抗体英文名：
anti-human IL-10 mAb 12G8, biotinylated
- 应用范围：
ELISA,ELISpot等
- 适应物种：
human
- 保质期：
18 months
- 库存：
大量
- 供应商：
欣博盛生物
- 克隆性：
单克隆抗体
- 规格：
1 mg
Intended use
This monoclonal antibody enables specific detection of human IL-10 in immunoassays such as ELISpot, FluoroSpot, and ELISA.Recommendation
12G8 is recommended as detection mAb in ELISpot, FluoroSpot, and ELISA in combination with capture mAb 9D7 (product code 3430-3).Product details
| Product | Anti-human IL-10 mAb (12G8), biotin |
| Application | ELISpot, FluoroSpot, ELISA |
| Analyte | IL-10 |
| Antibody | 12G8 |
| Conjugate | Biotin |
| Clonality | Monoclonal |
| Immunogen | Recombinant human IL-10 |
| Host | Rat |
| Isotype | IgG2a |
| Reactivity | Human |
| Specificity | Native and recombinant human IL-10 |
| Purification | Purified from in vitro cultures by protein G af-finity chromatography. |
| Biotinylation | Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin. |
| Concentration | 1 mg/ml |
| Supplied in | PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm). |
| Contents | Monoclonal antibody 12G8, biotinylated. Supplied at 1 mg/ml in PBS with 0.02% sodium azide. |
Shipping and Storage
| Shipping | Shipped at ambient temperature. |
| Storage | Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing. |
| Shelf life | At least 18 months from date of receipt. |
Mabtech是一家瑞典生物科技公司，1986年起源于斯德哥尔摩大学免疫学系，是ELISpot和T细胞检测技术的全球领先者。Mabtech公司以前沿科学研究和临床大规模筛选需求为向导，不断开发和生产出高质量的单克隆抗体和 ElISA、ELISpot、FluoroSpot试剂盒。并提供可拆分的ELISpot板和单独包装抗体等，满足了学术和工业不同需求。Mabtech产品主要包括抗体，ELISA Kits, ELISpot Kit, FluoroSpot Kits和附属产品。
更多详情请咨询Mabtech一级代理商-欣博盛生物科技
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文献和实验Dual- and Triple-Color Fluorospot
in the analysis, e.g., IL-10, rat mAb 9D7 is used for coating and tag (different from FITC and biotin)-labeled rat mAb 12G8 is used for detection. All mAbs from Mabtech, Nacka Strand, Sweden. 4. Secondary reagents for amplification: Mouse anti-FITC mAb
T-Cell Activation Using mAb to CD3
splenic T-cells and human peripheral T cells stimulated via CD3. Critical parameters include cell density, antibody titer and activation kinetics. Materials 1X sterile PBS Anti-mouse CD3e, Clone 145-2C11 (Functional Grade, Cat. No. 16
® TACS Agarose Column, 1 ml（Cat.no 6 - 6310 - 001） 10x Buffer CI, 85 ml （Cat.no 6 - 6320 - 085） Anti-hu CD235a Biotin mAb Anti-hu CD14 Biotin mAb Anti-hu CD3 Biotin mAb
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