产品说明: Clone 100 (See other available formats) Regulatory Status RUO Other Names B-Cell CLL/Lymphoma 2 (Bcl-2), Protein Phosphatase 1, Regulatory Subunit 50 Isotype Mouse IgG1
描述 Bcl-2是一种保守的抗凋亡蛋白,在正常免疫中发挥重要作用。它与其他Bcl-2家族成员形成同源二聚体或异二聚体。这种蛋白质通过控制线粒体融合和分裂来调节细胞凋亡。Bcl-2的磷酸化已显示出增强活性以允许对细胞外生长因子介导的信号作出反应。Bcl-2还受IRES、miR-15a、miR-16-1和RNA-BP核仁素的调节。在滤泡性淋巴瘤和一些弥漫性大B细胞淋巴瘤中经常观察到染色体18q21.3易位和Bcl-2过表达。 Bcl-2 is a conserved anti-apoptotic protein that plays important roles in normal immunity. It forms homodimers or heterodimers with other Bcl-2 family members. This protein regulates apoptosis through controlling mitochondrial fusion and fission. Phosphorylation of Bcl-2 has been shown to enhance activity to allow response to extracellular growth-factor-mediated signals. Bcl-2 is also regulated by IRES, miR-15a, miR-16-1 and RNA-BP nucleolin. Chromosome 18q21.3 translocation and overexpression of Bcl-2 is frequently observed in follicular lymphomas and some diffuse large B-cell lymphomas.
Product Details Isotype Control Alexa Fluor® 647 Mouse IgG1, κ Isotype Ctrl (FC) Verified Reactivity Human Antibody Type Monoclonal Host Species Mouse
Immunogen Synthetic peptide of amino acids 41-54 of human bcl-2 oncoprotein
Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. Application ICFC - Quality tested SB - Reported in the literature, not verified in house
Recommended Usage Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
Antigen Details Structure Two isoforms, 239 or 205 amino acids in length, with a predicted molecular weight of 26 kD or 22 kD and four ‘BH' domains.
Distribution Outer mitochondrial membrane, nuclear membrane, endoplasmic reticulum membrane.
Function Regulates apoptosis through controlling mitochondrial fusion and fission.
Interaction BAX, BAD, BAK, Bcl-X(L),EI24,APAF1, BBC3, BCL2L1, BNIPL, MRPL41, TP53BP2, FKBP8 BAG1, RAF1, EGLN3, G0S2, and BOP. Cell Type B cells Biology Area Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Chromatin Remodeling/Epigenetics, Immunology, Mitochondrial Function, Neuroscience, Signal Transduction, Transcription Factors, Ubiquitin/Protein Degradation