BioLegend 633512 PE/Cyanine7 a

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BioLegend 633512 PE/Cyanine7 anti-Bcl-2
产品简介：
产品品牌：BioLegend
产品货号：633512
产品名称：BioLegend 633512 PE/Cyanine7 anti-Bcl-2
产品规格：100 µg

产品说明：
Clone   BCL/10C4 (See other available formats)
Regulatory Status   RUO
Other Names   B-cell leukemia 2
Isotype   Mouse IgG1, κ

描述
Bcl-2（B细胞白血病2）是一种凋亡蛋白，是含有BH1-4结构域的Bcl-2家族成员。两种报道的异构体存在α=25kD；β=22kD。Bcl-2蛋白与其他Bcl-2家族成员形成同源或异源二聚体。Bcl-2分布于线粒体外膜、核膜和内质网。这种蛋白质通过控制线粒体膜通透性来阻断细胞凋亡死亡。Bcl-2的切割可以转化为促凋亡（通过BH4结构域的切割）。Bcl-2已被报道通过ROS调节细胞周期进程。该蛋白被ASK1/JNK1、PKC、ERKs和应激激活激酶磷酸化修饰，可以被泛素化。Bcl-2已被证明与Apaf-1、Raf-1、TP53BP2、胱天蛋白酶-3相互作用，并与Bax、Bad、Bak、Bcl-xL和Bag-1形成异二聚体。克隆BCL/10C4已被证明可用于小鼠和大鼠BCL-2蛋白的蛋白质印迹、免疫沉淀和免疫荧光。
Bcl-2 (B-cell leukemia 2) is an apoptotic protein and a member of the Bcl-2 family containing BH1-4 domains. Two reported isoforms exist α=25 kD; β=22 kD. The Bcl-2 protein forms homo- or hetero-dimers with other Bcl-2 family members. Bcl-2 is distributed in the outer mitochondrial membrane, the nuclear envelope, and the endoplasmic reticulum. This protein blocks apoptotic death by controlling mitochondrial membrane permeability. Cleavage of Bcl-2 can convert to pro-apoptotic (by cleavage of BH4 domain). Bcl-2 has been reported to regulate cell cycle progression via ROS. This protein is modified by ASK1/JNK1, PKC, ERKs, and stress-activated kinase phosphorylation and can be ubiquitinated. Bcl-2 has been shown to interact with Apaf-1, Raf-1, TP53BP2, caspase-3, and form heterodimers with Bax, Bad, Bak, Bcl-xL, and Bag-1. Clone BCL/10C4 has been shown to be useful for Western blotting, immunoprecipitation, and immunofluorescence of the mouse and rat Bcl-2 protein.

Product Details
Isotype Control   PE/Cyanine7 Mouse IgG1, κ Isotype Ctrl
Verified Reactivity   Mouse, Rat
Antibody Type   Monoclonal
Host Species   Mouse
Immunogen
N-terminal, Amino acid residues 61-76 of mouse Bcl-2

Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.

Concentration   0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application   ICFC - Quality tested

Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining using our nuclear factor staining protocol. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details
Structure
Apoptosis regulator proteins, Bcl-2 family, BH1-4 domains. Homo- or hetero-dimer; isoforms α, β 25 kD, 22 kD

Distribution
Outer mitochondrial membrane, intracellular membrane nuclear envelope, endoplasmic reticulum

Function
Blocks apoptotic death by controlling the mitochondrial membrane permeability. Converted to pro-apoptotic activity by cleavage of BH4 domain. Regulates cell cycle progression via ROS

Modification   Phosphorylation, Ubiquitination
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Neuroscience

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