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- 库存：
99
- 英文名：
PE anti-RPS6 Phospho (Ser235/Ser236)
- 供应商：
北京云肽生物科技有限公司
BioLegend 608604 PE anti-RPS6 Phospho (Ser235/Ser236)
北京云肽生物科技有限公司作为 BioLegend 的特约经销商，为广大客户提供原装正品，货期短，折扣好的服务准则。
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BioLegend 608604 PE anti-RPS6 Phospho (Ser235/Ser236)
产品简介：
产品品牌：BioLegend
产品货号：608604
产品名称：BioLegend 608604 PE anti-RPS6 Phospho (Ser235/Ser236)
产品规格：100 tests
产品说明：
Clone A17020B (See other available formats)
Regulatory Status RUO
Other Names S6, 40S ribosomal protein S6
Isotype Mouse IgG1, κ
描述
核糖体蛋白S6（RPS6）是小40S核糖体亚基的关键成分，也是真核核糖体中蛋白激酶的主要底物。响应于各种细胞刺激，如促有丝分裂刺激、胰岛素和增加的营养物质可利用性，上游激酶，如RSK和p70激酶在多个丝氨酸位点磷酸化RPS6。这些修饰促进7-甲基鸟嘌呤帽复合物的募集，从而促进翻译起始前复合物的组装并增加细胞蛋白质合成能力。RPS6在某些癌症中被证明是过度磷酸化的，磷酸化是糖尿病小鼠模型中胰腺β细胞大小和全身葡萄糖稳态功能的关键决定因素。
Ribosomal protein S6 (RPS6) is a key component of the small 40S ribosomal subunit and is the major substrate of protein kinases in eukaryotic ribosomes. In response to various cellular stimuli such as mitogenic stimulation, insulin, and increased nutrient availability, upstream kinases such as RSK and p70 kinases phosphorylate RPS6 at multiple serine sites. These modifications facilitate the recruitment of the 7-methylguanasine cap complex, thereby promoting the assembly of the translational pre-initiation complex and increased cellular protein synthesis capacity. RPS6 has been shown to be hyperphosphorylated in certain cancers, and phosphorylation is a critical determinant of pancreatic β–cell size and systemic glucose homeostasis function in diabeteic mouse models.
Product Details
Isotype Control PE Mouse IgG1, κ Isotype Ctrl (ICFC)
Verified Reactivity Human, Mouse
Antibody Type Monoclonal
Host Species Mouse
Immunogen
Synthetic peptide from human RPS6 phosphorylated at Serines 235 and 236
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application ICFC - Quality tested
Recommended Usage
Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Antigen Details
Structure
RPS6 is a 249 amino acid protein with a predicted molecular weight of 28.6 kD.
Distribution Cytosol, nucleus, ER, ubiquitous expression.
Function
Phosphorylation events at a cluster of serine residues (Ser 235, 236, 240, and 244) at the carboxy-terminus of RPS6 results in increased global translational efficiency. These sites are dephosphorylated during growth arrest.
Interaction
RPS6AK1 and RPS6AK3; other subunits of 40S ribosomal complex.
Biology Area
Cell Biology, Cell Proliferation and Viability, Protein Synthesis, Signal Transduction
Molecular Family Nuclear Markers, Phospho-Proteins
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文献和实验相关实验
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
Figure 18.20.1 The specificity of phospho‐motif antibodies. HeLa cells were serum‐starved for 24 hr prior to being stimulated with 50 ng/ml IGF‐1 or 100 nM PMA for 10 min. Lysates were immunoblotted with anti‐Akt/PKB phospho‐motif (left panel
Cell Surface Immunofluorescence Staining Protocol
实验试剂 Cell Staining Buffer [ Details:BioLegend Cat. #420201] Red Cell Lysis Buffer [ Details: BioLegend Cat. #420301] 7-AAD Viability Staining Solution[ Details: BioLegend Cat. #420403] TruStain FcX™[ Details: anti
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
3.1 and 3.6 ). This will provide a “specific activity” measurement relative to total protein in the cell, and is themost accurate measure of total GSK3 activity, or2. Immunoblot using antibodies to phospho-Ser21 (GSK3a)or phospho-Ser9 (GSK3b) (see Sections 3.1 –
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BioLegend 608604 PE anti-RPS6 Phospho (Ser235/Ser236)
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