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- 库存：
99
- 英文名：
APC/Cyanine7 anti-human CD11c
- 供应商：
北京云肽生物科技有限公司
BioLegend 337218 APC/Cyanine7 anti-human CD11c
北京云肽生物科技有限公司作为 BioLegend 的特约经销商，为广大客户提供原装正品，货期短，折扣好的服务准则。
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BioLegend 337218 APC/Cyanine7 anti-human CD11c
产品简介：
产品品牌：BioLegend
产品货号：337218
产品名称：BioLegend 337218 APC/Cyanine7 anti-human CD11c
产品规格：100 tests
产品说明：
Clone Bu15 (See other available formats)
Regulatory Status RUO
Workshop V S143
Other Names Integrin αx subunit, ITGAX, CR4, p150
Isotype Mouse IgG1, κ
描述
CD11c 是一种 145-150 kD I 型跨膜糖蛋白，也称为整合素 αx 和 CR4。CD11c 与整合素 β2 （CD18） 非共价结合，并在单核细胞/巨噬细胞、树突状细胞、粒细胞、NK 细胞以及 T 和 B 细胞亚群上表达。据报道，CD11c 通过与纤维蛋白原、CD54 和 iC3b 的相互作用在粘附和 CTL 杀伤中发挥作用。
CD11c is a 145-150 kD type I transmembrane glycoprotein also known as integrin αx and CR4. CD11c non-covalently associates with integrin β2 (CD18) and is expressed on monocytes/macrophages, dendritic cells, granulocytes, NK cells, and subsets of T and B cells. CD11c has been reported to play a role in adhesion and CTL killing through its interactions with fibrinogen, CD54, and iC3b.
Product Details
Isotype Control APC/Cyanine7 Mouse IgG1, κ Isotype Ctrl
Verified Reactivity Human
Antibody Type Monoclonal
Host Species Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application FC - Quality tested
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser Red Laser (633 nm)
Application Notes
Clone Bu15 has a different binding epitope than clone 3.9. The binding of Bu15 with CD11c is divalent cation independent. Additional reported applications (for the relevant formats of this clone) include: inhibition of CD11c mediated adhesion and stimulation of chemokine production by monocytes.
Additional Product NotesBioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Antigen Details
Structure
Integrin, type I transmembrane glycoprotein, associates with integrin β2 (CD18), 145-150 kD
Distribution
Myeloid, dendritic cells, NK cells, B cells and T cell subsets
Function
Adhesion, CTL killing Ligand Receptor: CD54, fibrinogen, iC3b, ICAM-1, ICAM-4 Antigen
Cell Type B cells, Dendritic cells, Neutrophils, NK cells, T cells
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family Adhesion Molecules, CD Molecules
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文献和实验相关实验
Cell Surface Immunofluorescence Staining Protocol
-CD4-Biotin, and anti-CD8-APC) and incubate on ice for 15-20 minutes in the dark. 9) Wash 2X with at least 2 ml of Cell Staining Buffer by centrifugation at 350 x g for 5 minutes. 1) If using a purified primary antibody, resuspend
Intracellular Cytokine Staining Protocol
are first activated in vitro, stained for surface antigens, as in the regular staining protocol, then fixed and permeabilized to allow for anti-cytokine antibodies to stain intracellularly. In vitro stimulation of cells is usually required for detection of cytokines
Intracellular Cytokine Staining Protocol
. In this protocol, cells are first activated in vitro, stained for surface antigens, as in the regular staining protocol, then fixed and permeabilized to allow for anti-cytokine antibodies to stain intracellularly. In vitro stimulation of cells is usually required
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BioLegend 337218 APC/Cyanine7 anti-human CD11c
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