BioLegend 330706 Alexa Fluor®

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BioLegend 330706 Alexa Fluor® 647 anti-human TRA-1-81
产品简介：
产品品牌：BioLegend
产品货号：330706
产品名称：BioLegend 330706 Alexa Fluor® 647 anti-human TRA-1-81
产品规格：100 tests

产品说明：
Clone  TRA-1-81 (See other available formats)
Regulatory Status  RUO
Other Names  Podocalyxin
Isotype  Mouse IgM, κ

描述
TRA-1-81抗体与由人胚胎干细胞（ESC）、胚胎生殖细胞（EG）和胚胎癌细胞表达的200-240kD细胞表面抗原反应。TRA-1-81在人ESC上的表达在分化时下调。进一步的研究表明，TRA-1-81是一种神经氨酸酶抗性碳水化合物表位，表达在具有CD34家族的粘蛋白样糖蛋白成员足角蛋白上。podocalytin是一种I型跨膜唾液蛋白，具有抗粘附性。
The TRA-1-81 antibody reacts with a 200-240 kD cell surface antigen expressed by human embryonic stem cells (ESC), embryonic germ cells (EG), and embryonal carcinoma cells. The expression of TRA-1-81 on human ESC is downregulated upon differentiation. Further studies have shown that TRA-1-81 is a neuroaminidase-resistant carbohydrate epitope expressed on podocalyxin, a member of mucin-like glycoprotein with CD34 family. Podocalyxin is a type I tranmembrane sialoprotein with anti-adhesion property.

Product Details
Isotype Control  Alexa Fluor® 647 Mouse IgM, κ Isotype Ctrl
Verified Reactivity  Human
Antibody Type  Monoclonal
Host Species  Mouse
Immunogen
Human embryonic carcinoma cell line 2102Ep cl.2A6

Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.

Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application   FC - Quality tested
ICC - Verified

Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser  Red Laser (633 nm)
Application Notes
Additional reported applications (for the relevant formats) include: immunofluorescence cell staining1, immunoprecipitation1, Western blotting1,2, ELISA1, and immunohistochemistry1 of *-fixed frozen tissue sections and formalin-fixed paraffin-embedded sections.

Antigen Details
Structure 
Mucin-like protein, CD34 family member, 200-240 kD
Distribution
embryonic stem cells (ESC), embryonic germ cells (EG), embryonal carcinoma cells
Function  Anti-adhesion
Cell Type  Embryonic Stem Cells
Biology Area  Cell Biology, Immunology, Stem Cells

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