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- 2025年11月08日
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99
- 英文名：
PE/Cyanine7 anti-mouse/human CD44 Recombinant
- 供应商：
北京云肽生物科技有限公司
北京云肽生物科技有限公司作为 BioLegend 的特约经销商，为广大客户提供原装正品，货期短，折扣好的服务准则。
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BioLegend 163607 PE/Cyanine7 anti-mouse/human CD44 Recombinant
产品简介：
产品品牌：BioLegend
产品货号：163607
产品名称：BioLegend 163607 PE/Cyanine7 anti-mouse/human CD44 Recombinant
产品规格： 25 µg
产品说明：
Clone QA19A43 (See other available formats)
Regulatory Status RUO
Other Names Hermes, Pgp1, H-CAM, or HUTCH
Isotype Rat IgG2a, κ
描述
CD44 在所有白细胞、内皮细胞、肝细胞和间充质细胞上均有表达。据报道，它是记忆细胞亚群的宝贵标记。Treg 细胞上的高 CD44 表达与通过高产量产生 IL-10 的有效抑制功能有关。CD44是一种粘附分子，参与白细胞附着在内皮细胞上并在内皮细胞上滚动，归巢于外周淋巴器官和炎症部位，以及白细胞聚集。
CD44 is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. It has been reported to be a valuable marker for memory cell subsets. High CD44 expression on Treg cells has been associated with potent suppressive function via high production of IL-10. CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation.
Product Details
Isotype Contro lPE/Cyanine7 Rat IgG2a, κ Isotype Ctrl
Verified Reactivity Mouse, Human
Antibody Type Recombinant
Host Species Rat
Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration 0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application FC - Quality tested
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
以下是 BioLegend 品牌部分产品，如需其他产品请联系本公司销售。
BioLegend 135525 PerCP/Cyanine5.5 anti-mouse CD115 (CSF-1R) AFS98 25 ug
BioLegend 135531 APC/Cyanine7 anti-mouse CD115 (CSF-1R) AFS98 25 ug
BioLegend 135532 APC/Cyanine7 anti-mouse CD115 (CSF-1R) AFS98 100 ug
BioLegend 135605 PE anti-mouse CD276 (B7-H3) MIH35 25 ug
BioLegend 135607 APC anti-mouse CD276 (B7-H3) MIH35 25 ug
BioLegend 135608 APC anti-mouse CD276 (B7-H3) MIH35 100 ug
BioLegend 135616 PerCP/Cyanine5.5 anti-mouse CD276 (B7-H3) MIH35 100 ug
BioLegend 135702 Purified anti-mouse CD39 5F2 100 ug
BioLegend 135802 Purified anti-mouse CD88 (C5aR) 20/70 100 ug
BioLegend 135805 PE anti-mouse CD88 (C5aR) 20/70 50 ug
BioLegend 135806 PE anti-mouse CD88 (C5aR) 20/70 200 ug
BioLegend 135816 Ultra-LEAF™ Purified anti-mouse CD88 (C5aR) 20/70 1 mg
BioLegend 135901 Purified anti-mouse CD140a APA5 25 ug
BioLegend 135902 Purified anti-mouse CD140a APA5 100 ug
BioLegend 135906 PE anti-mouse CD140a APA5 200 ug
BioLegend 135907 APC anti-mouse CD140a APA5 25 ug
BioLegend 135908 APC anti-mouse CD140a APA5 100 ug
BioLegend 135910 Biotin anti-mouse CD140a APA5 100 ug
BioLegend 135912 PE/Cyanine7 anti-mouse CD140a APA5 100 ug
BioLegend 135914 PerCP/Cyanine5.5 anti-mouse CD140a APA5 100 ug
BioLegend 135916 Brilliant Violet 605™ anti-mouse CD140a APA5 50 ug
BioLegend 136006 PE anti-mouse CD140b APB5 200 ug
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文献和实验The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
. 36. Resuspend the cells in OP9 medium, seed 5 x 105 cells per 10-cm dish containing ~80% confluent OP9 cells in 10 mL of OP9 medium, and add 5 ng/mL of recombinant human Flt-3L. Return the dish to the incubator. The number of 10-cm dishes
Intracellular Cytokine Staining Protocol
anti-cytokine antibodies with excess amounts of unlabeled antibody. Alternatively, recombinant cytokines can be used for blocking.eBioscience antibodies are available as Functional Grade (FG) Purified (sterile, endotoxin-tested, no azide), Purified
Intracellular Cytokine Staining Protocol
, it is common to block the directly-conjugated anti-cytokine antibodies with excess amounts of unlabeled antibody. Alternatively, recombinant cytokines can be used for blocking. eBioscience antibodies are available as Functional Grade (FG) Purified (sterile
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