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- 详细信息
- 文献和实验
- 技术资料
- 库存:
99
- 英文名:
PE/Cyanine7 anti-mouse CD55 (DAF)
- 供应商:
北京云肽生物科技有限公司
北京云肽生物科技有限公司作为BioLegend的特约经销商,为广大客户提供原装正品,货期短,折扣好的服务准则。
美国BioLegend公司位于美国加州,致力于为生物医药研究的各个前沿领域提供高质量的抗体及其它相关试剂。BioLegend虽然成立的时间不长,但它聚集了一批来自PharMingen公司的专业人士,建立了一支具有雄厚技术背景的专业队伍。通过其自身的先天优势,以及与世界知名实验室和研究所的良好合作关系,以合理的价格为广大客户提供高质量的免疫学和细胞生物学产品。BioLegend公司以其专业技术力量为保证,提供各种质量卓越的产品,并不断地开发新产品,以满足飞速发展的生命科学研究的需要。
BioLegend 131814 PE/Cyanine7 anti-mouse CD55 (DAF)
产品简介:
产品品牌:BioLegend
产品货号:131814
产品名称:BioLegend 131814 PE/Cyanine7 anti-mouse CD55 (DAF)
产品规格:100 µg
产品说明:
Clone RIKO-3 (See other available formats)
Regulatory Status RUO
Other Names Decay accelerating factor (DAF)
Isotype Armenian Hamster IgG
描述
CD55,也称为DAF(衰变加速因子),在红细胞、造血细胞和许多非止血细胞的表面表达。小鼠CD55有两种亚型,一种是GPI锚定的亚型,另一种是跨膜亚型。两者都得到RIKO-3的认可。CD55的功能是通过补体沉积来保护损伤。
CD55, also known as DAF (decay accelerating factor), is expressed on the surfaces of red blood cells, hematopoietic cells and many non-hemotopietic cells. There are two isoforms of mouse CD55, a GPI-anchored isofrom and a transmembrane isoform. Both are recognized by RIKO-3. The function of CD55 is to protect damage by complement deposition.
Product Details
Isotype Control PE/Cyanine7 Armenian Hamster IgG Isotype Ctrl
Verified Reactivity Mouse
Antibody Type Monoclonal
Host Species Armenian Hamster
Immunogen Transfected cells
Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration 0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application FC - Quality tested
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation LaserBlue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Antigen Details
Structure
60-70 kd single chain glycoprotein. While human CD55 contains only one CD55 gene, two distinct CD55 genes have been identified in the mouse, referred to as GPI-DAF and transmembrane(TM)-DAF. GPI-DAF is expressed only in testis.
Distribution
Red blood cells and most of the splenocytes, including B cells and T cells, and also many non-hematopoetic cells.
Function
Protect from complement attack by inhibition the deposition of C3 on antibody-sensitized cells.
Cell Type B cells, Erythrocytes, T cells
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroinflammation, Neuroscience
Molecular Family CD Molecules
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文献和实验Cell Surface Immunofluorescence Staining Protocol
. In the mouse, purified anti-mouse CD16/CD32 antibody specific for Fcγ R III/II (BioLegend Cat. #101302, clone 93) can be used to block nonspecific staining of antibodies. In this case, block Fc receptors by pre-incubating cells with 5-10 μg/ml purified anti-CD
() #交换 x 轴和 y 轴> p + ylim(0,15) #如果想截取某一段可以用此命令设置值域。> p + scale_x_continuous(breaks =seq(18,34,50) ) #设置刻度线位置> p + theme(axis.text.x = element_text(angle = 90,family = "Times",face = "italic",colour = "darkred",size=rel(0.9))) # angle = 90 设置字体角度, family
) RPMI 1640 medium without phenol red Primary antibodies: Lyve‐1, unconjugated (R&D) and PE‐conjugated CD31 (BioLegend
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