产品说明: Clone Poly5189 (See other available formats) Regulatory Status RUO Other Names Interleukin IL-17A, IL-17, Cytotoxic T lymphocyte-associated antigen 8 (CTLA-8) Isotype Goat Polyclonal Ig
描述 IL-17A是IL-17家族的创始成员,该家族由六种结构相关的促炎细胞因子组成。IL-17A由活化的CD4+Th17细胞亚群分泌,在多种细胞上引发多种生物活性,包括:诱导上皮、内皮或成纤维细胞产生IL-6、IL-8、G-CSF和PGE2;成纤维细胞表面ICAM-1表达的增强;κB的激活和T细胞增殖的共刺激。最近的研究表明,在小鼠中,活化的分泌IL-17的CD4+辅助性T细胞(Th17细胞)介导一种自身免疫性关节炎,该关节炎在临床和免疫学上类似于类风湿性关节炎(RA)。人IL-17A分别与大鼠IL-17A、小鼠IL-17A和赛米里疱疹病毒(HVS)ORF13基因编码的蛋白质显示63%、63%和72%的氨基酸序列同一性。 IL-17A is the founding member of the IL-17 family, a group of six structurally related pro-inflammatory cytokines. IL-17A, secreted by activated CD4+ Th17 cell subpopulation, elicits multiple biological activities on a variety of cells including: the induction of IL-6, IL-8, G-CSF, and PGE2 production in epithelial, endothelial or fibroblasts; the enhancement of surface expression of ICAM-1 in fibroblasts; activation of NF-κB and costimulation of T cell proliferation. Recent studies demonstrated that, in mice, activated IL-17-secreting CD4+ helper T cells (Th17 cells) mediate an autoimmune arthritis that clinically and immunologically resembles rheumatoid arthritis (RA). Human IL-17A shows 63%, 63%, and 72% amino acid sequence identity to rat IL-17A, mouse IL-17A, and a protein encoded by the ORF13 gene of herpesvirus Saimiri (HVS), respectively.
Product Details Verified Reactivity Human Antibody Type Polyclonal Host Species Goat Immunogen Recombinant human IL-17A, amino acids Ile20-Ala155 (Accession # NM_002190) was expressed in E. coli.
Preparation The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions. Concentration 0.2 mg/ml
Storage & Handling The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze. Application ELISA Detection - Quality tested
Recommended Usage Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.25-1 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance. To obtain a linear standard curve, serial dilutions of IL-17A recombinant protein ranging from 1000 to 15.6 pg/ml are recommended.
Antigen Details Structure Cytokine; dimer; ~15 kD (Mammalian) Bioactivity Secretion of IL-6, IL-8, G-CSF, prostaglandin E2 by epithelial, endothelial or fibroblastic cells; stimulates cell migration, cord formation, and IL-6 secretion by stromal cells Cell Sources Activated T cell subpopulation Cell Targets Fibroblasts, epithelial and endothelial cells, stromal cells
Cross In sandwich ELISA, cross-reactivity with human IL-17F is negligible and no cross-reactivity with mouse IL-17A is present. Receptors IL-17R Biology Area Cell Biology, Neuroinflammation, Neuroscience Molecular Family Cytokines/Chemokines