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- 2025年10月23日
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- 文献和实验
- 技术资料
- 库存：
99
- 英文名：
PE anti-mouse Siglec H
- 供应商：
北京云肽生物科技有限公司
北京云肽生物科技有限公司作为BioLegend的特约经销商，为广大客户提供原装正品，货期短，折扣好的服务准则。
美国BioLegend公司位于美国加州，致力于为生物医药研究的各个前沿领域提供高质量的抗体及其它相关试剂。BioLegend虽然成立的时间不长，但它聚集了一批来自PharMingen公司的专业人士，建立了一支具有雄厚技术背景的专业队伍。通过其自身的先天优势，以及与世界知名实验室和研究所的良好合作关系，以合理的价格为广大客户提供高质量的免疫学和细胞生物学产品。BioLegend公司以其专业技术力量为保证，提供各种质量卓越的产品，并不断地开发新产品，以满足飞速发展的生命科学研究的需要。
BioLegend 129605 PE anti-mouse Siglec H
产品简介：
产品品牌：BioLegend
产品货号：129605
产品名称：BioLegend 129605 PE anti-mouse Siglec H
产品规格：25 µg
产品说明：
Clone 551 (See other available formats)
Regulatory Status RUO
Other Names Sialic acid-binding immunoglobulin-like lectin
Isotype Rat IgG1, κ
描述
Siglec-H是CD33相关Siglec家族的成员，其主要在先天免疫系统中表达。据报道Siglec-H在浆细胞样树突状细胞上特异性表达。Siglec-H依赖于衔接蛋白DAP12（12kD的DNAX激活蛋白）进行表面表达和信号转导。与该抗体的交联可以选择性地抑制TLR9连接后IPC产生干扰素α。
The Siglec-H is a member of CD33-related Siglec family which mostly expressed in the innate immune system. Siglec-H is reported to be specifically expressed on plasmacytoid dendritic cells. Siglec-H depends on adaptor protein DAP 12 (DNAX-activating protein of 12 kD) for surface expression and signal transduction. Cross-linking with this antibody can selectively inhibit interferon-alpha production by IPCs following TLR9 ligation.
Product Details
Isotype Control PE Rat IgG1, κ Isotype Ctrl
Verified Reactivity Mouse
Antibody Type Monoclonal
Host Species Rat
Immunogen Siglec-H-Fc recombinant fusion protein
Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration 0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application FC - Quality tested
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Antigen Details
Structure Sialic acid-binding immunoglobulin-like lectins
Distribution
Siglec-H is expressed exclusively on natural interferon-producing cells (IPCs), also called plasmacytoid dendritic cells in spleen, blood and lymph nodes. The IPCs are characterized by a complex phenotype (CD11c+, Ly-6C+, B220+, CD11b-, CD8+/-).
Function
Siglec-H associates with adaptor protein DAP12 for cell signaling, which reduces type 1 IFN response of IPC to CpG.
Ligand/Receptor Sialic acid
Cell Type Dendritic cells
Biology Area Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family CD Molecules, Siglec Molecules
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