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- 详细信息
- 文献和实验
- 技术资料
- 库存:
99
- 英文名:
PE anti-mouse H-2
- 供应商:
北京云肽生物科技有限公司
北京云肽生物科技有限公司作为BioLegend的特约经销商,为广大客户提供原装正品,货期短,折扣好的服务准则。
美国BioLegend公司位于美国加州,致力于为生物医药研究的各个前沿领域提供高质量的抗体及其它相关试剂。BioLegend虽然成立的时间不长,但它聚集了一批来自PharMingen公司的专业人士,建立了一支具有雄厚技术背景的专业队伍。通过其自身的先天优势,以及与世界知名实验室和研究所的良好合作关系,以合理的价格为广大客户提供高质量的免疫学和细胞生物学产品。BioLegend公司以其专业技术力量为保证,提供各种质量卓越的产品,并不断地开发新产品,以满足飞速发展的生命科学研究的需要。
BioLegend 125506 PE anti-mouse H-2
产品简介:
产品品牌:BioLegend
产品货号:125506
产品名称:BioLegend 125506 PE anti-mouse H-2
产品规格:100 µg
产品说明:
Clone M1/42 (See other available formats)
Regulatory Status RUO
Other Names Mouse major histocompatibility complex (MHC) H-2
Isotype Rat IgG2a, κ
描述
M1/42抗体与来自a、b、d、j、k、s和u单倍型小鼠的有核细胞上表达的H-2MHC I类同种抗原反应(Stallcup,KC等人,1981)。MHC I类参与向表达CD3/TCR和CD8蛋白的T细胞的抗原呈递。
The M1/42 antibody reacts with the H-2 MHC class I alloantigens expressed on nucleated cells from mice of the a, b, d, j, k, s, and u haplotypes (Stallcup, KC et al, 1981). MHC class I is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins.
Product Details
Isotype Control PE Rat IgG2a, κ Isotype Ctrl
Verified Reactivity Mouse
Antibody Type Monoclonal
Host Species Rat
Immunogen C57BL/10 spleen cells
Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration 0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application FC - Quality tested
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for other applications.
Excitation Laser Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Antigen Details
Structure 45kd heavy chain non-covalently associated with 12 kd beta-2 microglobulin
Distribution All nucleated cells
Function Present antigenic peptide to CD8+ cytotoxic T cells
Ligand/Receptor MHC/peptide
Biology Area Immunology
Molecular Family MHC Antigens
以下是BioLegend 品牌部分产品,如需其他产品请联系本公司销售。
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文献和实验T-cell dependent antigen specific in situ staining (Pe)
6- Wash twice in TBS-T. 7- counterstain with rabbit anti B-Pe 1:2000 (0.5 µg/ml Cortex Biochem). You may add 1% mouse serum to the anti-Pe antibodies, unless you plan to use mouse monoclonal further (see below). 8- wash twice in TBS-T. 9- block
Cell Surface Immunofluorescence Staining Protocol
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Appropriate mouse strain Sodium pentobarbital Solution of 2.5 µCi of [14 C]cholesterol, 5 µCi [3 H
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