BioLegend 121216 PE/Cyanine5 a

北京云肽生物科技有限公司作为BioLegend的特约经销商，为广大客户提供原装正品，货期短，折扣好的服务准则。

美国BioLegend公司位于美国加州，致力于为生物医药研究的各个前沿领域提供高质量的抗体及其它相关试剂。BioLegend虽然成立的时间不长，但它聚集了一批来自PharMingen公司的专业人士，建立了一支具有雄厚技术背景的专业队伍。通过其自身的先天优势，以及与世界知名实验室和研究所的良好合作关系，以合理的价格为广大客户提供高质量的免疫学和细胞生物学产品。BioLegend公司以其专业技术力量为保证，提供各种质量卓越的产品，并不断地开发新产品，以满足飞速发展的生命科学研究的需要。

BioLegend 121216  PE/Cyanine5 anti-mouse CD43 Activation-Associated Glycoform
产品简介：
产品品牌：BioLegend
产品货号：121216
产品名称：BioLegend 121216 PE/Cyanine5 anti-mouse CD43 Activation-Associated Glycoform
产品规格： 100 µg

产品说明：
Clone  1B11 (See other available formats)
Regulatory Status  RUO
Other Names  Ly-48, Leukosialin, Sialophorin, Leukocyte Sialoglycoprotein, gp115
Isotype  Rat IgG2a, κ

描述
CD43，也称为Ly-48、白细胞唾液蛋白、唾液蛋白、白细胞Sialophorin和gp115，是一种I型跨膜糖蛋白的大单链，具有丰富的O-糖基化和唾液酸化位点。由于可变的糖基化和唾液酸化，已经鉴定出CD43的两种亚型。CD43的115kD糖型在大多数造血细胞上表达，包括T淋巴细胞、NK细胞、单核细胞、粒细胞、血小板和CD5+B细胞。它不存在于静止的B细胞和红细胞上。而130kD糖型被认为是主要在髓系细胞、前B细胞和活化的T细胞上表达的活化相关形式。据报道，CD43与CD54和Siglec-1结合。CD43在细胞粘附和抗粘附、共刺激T细胞活化和存活以及诱导T细胞和造血祖细胞凋亡中发挥双重作用。1B11抗体与CD43的活化相关糖型反应。1B11识别的表位也与去分析的CD45RB共享。该抗体可用于效应CD8 T细胞和记忆T细胞的分化。
CD43, also known as Ly-48, Leukosialin, Sialophorin, Leukocyte Sialoglycoprotein, and gp115, is a large single chain of type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites. Due to variable glycosylation and sialylation, two isoforms of CD43 have been identified. The 115 kD glycoform of CD43 is expressed on most hematopietic cells including T lymphocytes, NK cells, monocytes, granulocytes, platelets, and CD5+ B cells. It is not present on resting B cells and erythrocytes. While the 130 kD glycoform is thought to be activation-associated form primarily expressed on myeloid cells, pre-B cells, and activated T cells. It has been reported that CD43 binds to CD54 and Siglec-1. CD43 plays dual roles in cell adhesion and anti-adhesion, as well as costimulation of T cell activation and survival, and induction of apoptosis of T cells and hematopoietic progenitors. The 1B11 antibody reacts with the activation-associated glycoform of CD43. The epitope recognized by 1B11 is also shared with desialylated CD45RB. This antibody is useful for differentiation of effector CD8 T cells and memory T cells.

Product Details
Isotype Control  PE/Cyanine5 Rat IgG2a, κ Isotype Ctrl
Verified Reactivity  Mouse
Antibody Type  Monoclonal
Host Species  Rat
Immunogen  Mouse WEHI 274.3 myeloid tumor cells
Formulation  Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation  The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine5 under optimal conditions.
Concentration  0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application  FC - Quality tested
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation  Laser  Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, Western blotting1,2, and immunohistochemistry of *-fixed frozen sections

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy5 to PE/Cyanine5. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine5 products. Please contact Technical Service if you have any questions.

Antigen Details
Structure   
Single chain of type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites, 115 kD and 130 kD glycoforms with different glycosylation and sialylation
Distribution
Most hematopoietic cells including peripheral T cells, NK cells, granulocytes, monocytes, thymocytes, platelets, plasma cells, CD5+ B-1 cells and activated B cells, and hemotopoietic progenitor cells.
Function
Play a dual roles in cell-cell adhesion and anti-adhesion, costimulation of cell activation and survival, induce apoptosis of T cells and hematopoietic progenitors
Ligand/Receptor  CD54, Siglec-1
Cell Type     
B cells, Granulocytes, Hematopoietic stem and progenitors, Monocytes, NK cells, Plasma cells, Platelets, T cells, Thymocytes
Biology Area  Immunology
Molecular Family  Adhesion Molecules, CD Molecules

以下是BioLegend  品牌部分产品，如需其他产品请联系本公司销售。
BioLegend 104723 Pacific Blue™ anti-mouse CD80   16-10A1    25 ug
BioLegend 104724 Pacific Blue™ anti-mouse CD80   16-10A1    100 ug
BioLegend 104725 Brilliant Violet 421™ anti-mouse CD80    16-10A1    125 uL
BioLegend 104726 Brilliant Violet 421™ anti-mouse CD80    16-10A1    500 uL
BioLegend 104729 Brilliant Violet 605™ anti-mouse CD80    16-10A1    125 uL
BioLegend 104731 Brilliant Violet 650™ anti-mouse CD80    16-10A1    125 uL
BioLegend 104732 Brilliant Violet 650™ anti-mouse CD80    16-10A1    50 ug
BioLegend 104735 Purified anti-mouse CD80 (Maxpar® Ready)     16-10A1    100 ug
BioLegend 104737 PE/Dazzle™ 594 anti-mouse CD80    16-10A1    25 ug
BioLegend 104738 PE/Dazzle™ 594 anti-mouse CD80    16-10A1    100 ug
BioLegend 104740 APC/Fire™ 750 anti-mouse CD80 16-10A1    100 ug
BioLegend 104741 Brilliant Violet 510™ anti-mouse CD80    16-10A1    50 ug
BioLegend 104743 Brilliant Violet 711™ anti-mouse CD80    16-10A1    50 ug
BioLegend 104748 Ultra-LEAF™ Purified anti-mouse CD80  16-10A1    1 mg
BioLegend 104754 Alexa Fluor® 594 anti-mouse CD80  16-10A1    100 ug
BioLegend 104759 PE/Fire™ 640 anti-mouse CD80   16-10A1    25 ug
BioLegend 104903 Biotin anti-mouse/rat CD81   Eat-2   50 ug
BioLegend 104905 PE anti-mouse/rat CD81  Eat-2   50 ug
BioLegend 104906 PE anti-mouse/rat CD81  Eat-2   200 ug
BioLegend 104909 APC anti-mouse/rat CD81     Eat-2   25 ug
BioLegend 104910 APC anti-mouse/rat CD81     Eat-2   100 ug
BioLegend 104912 PerCP/Cyanine5.5 anti-mouse/rat CD81 Eat-2   100 ug