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- 库存:
99
- 英文名:
PE/Cyanine7 anti-mouse CD252 (OX40 Ligand)
- 供应商:
北京云肽生物科技有限公司
北京云肽生物科技有限公司作为BioLegend的特约经销商,为广大客户提供原装正品,货期短,折扣好的服务准则。
美国BioLegend公司位于美国加州,致力于为生物医药研究的各个前沿领域提供高质量的抗体及其它相关试剂。BioLegend虽然成立的时间不长,但它聚集了一批来自PharMingen公司的专业人士,建立了一支具有雄厚技术背景的专业队伍。通过其自身的先天优势,以及与世界知名实验室和研究所的良好合作关系,以合理的价格为广大客户提供高质量的免疫学和细胞生物学产品。BioLegend公司以其专业技术力量为保证,提供各种质量卓越的产品,并不断地开发新产品,以满足飞速发展的生命科学研究的需要。
BioLegend 108814 PE/Cyanine7 anti-mouse CD252 (OX40 Ligand)
产品简介:
产品品牌:BioLegend
产品货号:108814
产品名称:BioLegend 108814 PE/Cyanine7 anti-mouse CD252 (OX40 Ligand)
产品规格:100 µg
产品说明:
Clone RM134L (See other available formats)
Regulatory Status RUO
Other Names OX-40L, CD134L, CD252, TNFSF4
Isotype Rat IgG2b, κ
描述
CD252是TNF超家族的35kD成员,也被称为OX40配体、OX40L和CD134L。CD252在活化的B细胞和抗原呈递细胞上表达。CD252与OX40抗原(CD134)相互作用,主要在活化的T细胞上表达,以增加增殖和IL-2的产生,并增强活化的B细胞的增殖和免疫球蛋白分泌。
CD252 is a 35 kD member of the TNF superfamily and is also known as OX40 ligand, OX40L, and CD134L. The CD252 is expressed on activated B cells and antigen presenting cells. CD252 interacts with OX40 antigen (CD134), expressed predominantly on activated T cells to increase proliferation and IL-2 production and to enhance proliferation and immunoglobulin secretion in activated B cells.
Product Details
Isotype Control PE/Cyanine7 Rat IgG2b, κ Isotype Ctrl
Verified Reactivity Mouse
Antibody Type Monoclonal
Host Species Rat
Immunogen Rat NRK-52E cell line transfected with mouse OX-40L
Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration 0.2 mg/ml
Storage & Handling The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application FC - Quality tested
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes
The RM134L antibody can block the costimulatory activity of OX40L. Additional reported applications (for the relevant formats) include: immunohistochemical staining of *-fixed frozen sections3, and in vivo and in vitro blocking of OX-40L-OX-40 functional interaction1,2,4. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 108808).
Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Antigen Details
Structure NGF/TNF superfamily, 35 kD
Distribution Activated B cells, antigen presenting cells
Function B-cell/T-cell interaction,costimulation
Ligand/Receptor OX40 (CD134)
Cell Type Antigen-presenting cells, B cells
Biology Area Costimulatory Molecules, Immunology
Molecular Family CD Molecules, Immune Checkpoint Receptors
以下是BioLegend 品牌部分产品,如需其他产品请联系本公司销售。
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BioLegend 101251 Brilliant Violet 421™ anti-mouse/human CD11b M1/70 50 ug
BioLegend 101254 Alexa Fluor® 594 anti-mouse/human CD11b M1/70 100 ug
BioLegend 101256 PE/Dazzle™ 594 anti-mouse/human CD11b M1/70 100 ug
BioLegend 101257 Brilliant Violet 605™ anti-mouse/human CD11b M1/70 50 ug
BioLegend 101259 Brilliant Violet 650™ anti-mouse/human CD11b M1/70 50 ug
BioLegend 101261 APC/Fire™ 750 anti-mouse/human CD11b M1/70 25 ug
BioLegend 101262 APC/Fire™ 750 anti-mouse/human CD11b M1/70 100 ug
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BioLegend 101302 Purified anti-mouse CD16/32 93 500 ug
BioLegend 101303 Biotin anti-mouse CD16/32 93 50 ug
BioLegend 101305 FITC anti-mouse CD16/32 93 50 ug
BioLegend 101306 FITC anti-mouse CD16/32 93 500 ug
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文献和实验Cell Surface Immunofluorescence Staining Protocol
. In the mouse, purified anti-mouse CD16/CD32 antibody specific for Fcγ R III/II (BioLegend Cat. #101302, clone 93) can be used to block nonspecific staining of antibodies. In this case, block Fc receptors by pre-incubating cells with 5-10 μg/ml purified anti-CD
) A431 cells. At 48 h after infection, these cells were stained with ten different primary antibodies (5 g/ml) specific for different cell surface markers: anti-CD15 (clone 2F3; BD, PharMingen, San Diego, CA), anti-ERBB-2 (clone SP77; ref. 5), anti-ERBB
) RPMI 1640 medium without phenol red Primary antibodies: Lyve‐1, unconjugated (R&D) and PE‐conjugated CD31 (BioLegend
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