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- 详细信息
- 文献和实验
- 技术资料
- 库存:
99
- 英文名:
I-Block Protein-Based Blocking Reagent
- 供应商:
北京云肽生物科技有限公司
我们北京云肽生物科技有限公司作为赛默飞Invitrogen公司的特约经销商为客户提供,正规进口,保证原装正品,货期稳定,折扣好的服务标准。
赛默飞世尔科技公司(Thermo Fisher Scientific)赛默飞世尔科技是全球科学服务领域的lingdaozhe(纽约证交所代码:TMO),致力于帮助客户使世界更健康,更清洁,更安全。
公司借助于Thermo Scientific、Life Technologies、Fisher Scientific和Unity™ Lab Services四个首要品牌,帮助客户解决在分析化学领域从常规的测试到复杂的研发项目中所遇到的各种挑战。
Thermo Scientific能够为客户提供一整套包括高端分析仪器、实验室装备、软件、服务、耗材和试剂在内的实验室综合解决方案。Thermo Fisher Scientific将努力为客户提供jiwei便 捷的采购方案,为科研的飞速发展不断地改进工艺技术,提升客户价值。
赛默飞InvitrogenT2015 I-Block Protein-Based Blocking Reagent /I-Block蛋白封闭剂,产品简介:
产品品牌:赛默飞Invitrogen
产品货号:T2015
产品名称:I-Block Protein-Based Blocking Reagent /I-Block蛋白封闭剂
产品规格:30 g
赛默飞InvitrogenT2015 I-Block Protein-Based Blocking Reagent /I-Block蛋白封闭剂,产品说明:
I-Block 试剂是一种高纯度基于酪蛋白的封闭试剂。与干乳和 BSA 相比具有更出色的封闭能力。与其他基于酪蛋白的封闭试剂不同,I-Block 试剂基本上无生物素。在使用 Tropix 底物和碱性磷酸酶偶联物的测定中检测 I-Block 试剂,在基于膜和免疫检测应用中用作封闭试剂。
建议的工作浓度为 0.2% (w/v),用于检测中性或带正电荷尼龙膜上的核酸以及在膜(硝酸纤维素、PVDF 或中性尼龙)上进行免疫检测和蛋白检测。对于带正电荷尼龙膜(如 TropilonPlus 膜)上的蛋白检测,建议浓度为 3%。I-Block 封闭溶液在加热 (40-50°C) 下在 Tris 或磷酸盐缓冲盐水缓冲液中制备。
这种基于酪蛋白的封闭试剂可与 Western-Light、Western-Light Plus、Western-Star、ELISA-Light、Southern-Light 和 Southern-Star 检测系统配合使用。
以下是Thermo Scientific品牌部分产品订购信息,如需其他产品请联系本公司客服
品牌 货号 产品名称 规格
Thermo Scientific 37571 Pierce Protein-Free T20 (TBS) Blocking Buffer/T20(TBS)封闭缓冲液/封闭液 1 L
Thermo Scientific 37573 Pierce Protein-Free T20 (PBS) Blocking buffer/T20(TBS)封闭缓冲液/封闭液 1 L
Thermo Scientific 37570 Pierce Protein-Free (TBS) Blocking Buffer/无蛋白(TBS)封闭缓冲液/封闭液 1 L
Thermo Scientific 37585 Pierce Protein-Free (TBS) Blocking Buffer/无蛋白(TBS)封闭缓冲液/封闭液 100 mL
Thermo Scientific 37572 Pierce Protein-Free (PBS) Blocking buffer/无蛋白(PBS)封闭缓冲液/封闭液 1 L
Thermo Scientific 37584 Pierce Protein-Free (PBS) Blocking buffer/无蛋白(PBS)封闭缓冲液/封闭液 100 mL
Thermo Scientific 37575 Pierce Fast Blocking Buffer/快速封闭缓冲液/封闭液 500 mL
Thermo Scientific 37576 Pierce Fast Blocking Buffer/快速封闭缓冲液/封闭液 100 mL
Thermo Scientific 37545 SuperBlock (TBS) Blocking Buffer Dry Blend SuperBlock /封闭液 5 packs
Thermo Scientific 37520 Blocker BSA (10X) in TBS/Blocker BSA(10X)TBS封闭缓冲液/封闭液 125 mL
Thermo Scientific 37525 Blocker BSA in PBS (10X)/Blocker(10X) BSA封闭缓冲液/封闭液 100 mL
Thermo Scientific 37528 Blocker Casein in PBS/酪蛋白封闭缓冲液/封闭液 1 L
Thermo Scientific 37582 Blocker Casein in PBS/酪蛋白封闭缓冲液/封闭液 100 mL
Thermo Scientific 37532 Blocker Casein in TBS/酪蛋白封闭缓冲液/封闭液 1 L
Thermo Scientific 37583 Blocker Casein in TBS/酪蛋白封闭缓冲液/封闭液 100 mL
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文献和实验Blocking of unwanted non-specific staining
, 1997). Incubate for 10 min. minimum. (Use the yolk as directed in Cucinait.com ) NOTE: this passage may be omitted, however subsequent double staining with a biotin based method may need endogenous biotin blocking. 3- Wash once in TBS-T.
BLOCK-iT Fluorescent Oligo as RNAi Transfection Control
-iT™ Fluorescent Oligo for Cationic Lipid-Mediated Transfection You may use the BLOCK-iT™ Fluorescent Oligo (20 µM or 1 mM stock) with any cationic lipid-based transfection reagent suitable for delivery of Stealth™ RNAi, siRNA, or Dicer-generated siRNA
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
. A small block made of polyvinyl chloride (2.5 x 2.5 x 1.5 cm) is attached to a flexible Teflon ribbon as shown. The block should hang ~2-3 mm above the plane of the holding block (also made of polyvinyl chloride). A piece of filter paper (i.e., Whatman 3MM
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