STEMCELL Technologies 09704 HemaTox™ Myeloid Kit/HemaTox™髓系分化检测试剂盒,产品说明: HemaTox™ Myeloid Kit is intended for the measurement of drug toxicity on the proliferation and myeloid-specific differentiation of human hematopoietic stem and progenitor cells (HSPCs) in a liquid culture-based 96-well plate format. HemaTox™ Myeloid Kit includes a specialized serum-free culture medium and 100X supplement. Complete HemaTox™ Myeloid Medium (HemaTox™ Myeloid Medium + HemaTox™ Myeloid 100X Supplement) promotes the proliferation of human CD34+ HSPCs and their differentiation into myeloid cells during a 7-day culture period. After culture, the cells can be counted and assessed for expression of myeloid markers such as CD13, CD14, and CD15 using flow cytometry or other methods. HemaTox™ Myeloid Kit may be used on its own or in combination with HemaTox™ Erythroid Kit (Catalog #09701) or HemaTox™ Megakaryocyte Kit (Catalog #09707) to assess lineage-specific drug toxicity in parallel. Each kit contains sufficient medium and supplement for testing up to 160 different conditions (triplicate wells per condition, 200 µL per well) in 5 x 96-well plates. HemaTox™髓系试剂盒用于在基于液体培养基的96孔板中测定药物对人造血干细胞和祖细胞(HSPCs)的增殖和髓系特异性分化的毒性。 HemaTox™髓系试剂盒包括一个专门的无血清培养基和100X补充剂。完整的HemaTox™髓样培养基(HemaTox™髓样培养基+ HemaTox™髓样100X增补剂)可促进人类CD34 + HSPCs的扩增,并在培养7天的过程中分化为髓样细胞。培养后,可用流式仪或其他方法对细胞进行计数和评估骨髓标志物(如CD13,CD14和CD15)的表达。HemaTox™髓系试剂盒可单独使用或与HemaTox™Erythroid试剂盒(货号#09701)或HemaTox™巨核细胞试剂盒(货号#09707),同时评估谱系特异性药物毒性。每个试剂盒含有足够的培养基和补充剂,用于检测多达160种不同条件(每种条件一式三份,每孔200μL) ×96孔板。