- ￥895 - 11225
- 翌圣生物（Yeasen）
- 93558ES
- 上海
- 2025年12月05日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
现货
- 英文名：
Recombinant Human PD-1/PDCD1(His Tag)
- 保质期：
2年
- 供应商：
翌圣生物科技（上海）股份有限公司
- 保存条件：
-20度
- 规格：
100 μg(93558ES60)/500 μg(93558ES76)/25μg(93558ES25)
| 规格： | 100 μg(93558ES60) | 产品价格： | ￥3225.00 |
|---|---|---|---|
| 规格： | 500 μg(93558ES76) | 产品价格： | ￥11225.00 |
| 规格： | 25μg(93558ES25) | 产品价格： | ￥895.00 |
产品描述
Programmed Death-1 receptor, also known as PD-1 and CD279, is a receptor expressed on T cells responsible for modulating T cell activation. Like CTLA4, PD-1 is classified as an immune checkpoint inhibitory receptor. Mature human PD-1 consists of an extracellular region (ECD) with one immunoglobulin-like Vtype domain, a transmembrane domain, and a cytoplasmic region. The mature ECD of human PD-1 shares 61% amino acid sequence identity with mouse PD-1 ECD. PD1 is expressed on activated T cells, B cells, monocytes, and dendritic cells while PD-L1 expression is constitutive on the same cells and also on nonhematopoietic cells such as lung endothelial cells and hepatocytes. Ligation of PD-L1 with PD-1 induces co-inhibitory signals on T cells promoting their apoptosis, anergy, and functional exhaustion.
产品性质
| 别名（Synonyms） |
PDCD1, PD1, CD279, SLEB2 |
| Uniprot No. |
|
| 表达区间及表达系统（Source） |
HEK293 Cells-derived Human PD-1 / PDCD1 Protein, Leu25-Gln167 with His tag at the C-terminal |
| 分子量(Molecular Weight) |
Approximately 17.0 kDa. Due to glycosylation, the protein migrates to 38-50 kDa based on Tris-Bis PAGE result. |
| 外观(Physical Appearance） |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| 纯度（Purity） |
> 95% as determined by SDS-PAGE and HPLC |
| 活性（Activity） |
ELISA Data: Immobilized Human PD-1, His Tag at 0.5 μg/mL (100 μL/Well). Dose response curve for Anti-PD-1 Antibody, hFc Tag with the EC50 of 45.0 ng/mL determined by ELISA. |
| 内毒素（Endotoxin） |
< 1.0 EU per 1μg of the protein by the LAL method. |
| 制剂（Formulation） |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| 复溶（Reconstitution） |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL` is recommended (usually we use 1mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
运输与保存方法
冰袋运输。-20℃至-80℃保存，一年有效期。
复溶后，-20 至 -80°C，在未开封状态下保存 3-6 个月。复溶后，2-8°C 保存 2-7 天。
建议第一次使用时分装冻存，避免反复冻融。
注意事项
1. 避免反复冻融。
2. 为了您的安全和健康，请穿实验服并戴一次性手套操作。
3. 本产品仅作科研用途！
产品数据
HB220629
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