Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-9 (gelatinase B) can degrade a broad range of substrates including gelatin, collagen types IV and V, elastin and proteoglycan core protein. It is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP-9 is produced by keratinocytes, monocytes, macrophages and PMN leukocytes. MMP-9 is present in most cases of inflammatory responses. Structurally, MMP-9 maybe be divided into five distinct domains: a pro-domain which is cleaved upon activation, a gelatin-binding domain consisting of three contiguous fibronectin type II units, a catalytic domain containing the zinc binding site, a proline-rich linker region, and a carboxyl terminal hemopexin-like domain.
产品性质
别名(Synonyms)
MMP-9; GELB; CLG4B; MANDP2; Gelatinase B
Uniprot No.
P14780
表达区间及表达系统(Source)
Recombinant Human MMP-9 Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Ala20-Asp707.
分子量(Molecular Weight)
The protein has a predicted MW of 79.3 kDa. Due to glycosylation, the protein migrates to 85-100 kDa based on Tris-Bis PAGE result.
外观(Physical Appearance)
Sterile Filtered White lyophilized (freeze-dried) powder.
纯度(Purity)
> 95% as determined by SDS-PAGE and HPLC
活性(Activity)
ELISA Data:Immobilized Human MMP-9, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-MMP-9 Antibody, hFc Tag with the EC50 of 17.4ng/ml determined by ELISA.
内毒素(Endotoxin)
< 1.0 EU per 1μg of the protein by the LAL method.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
复溶(Reconstitution)
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.