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- 询价记录
- 文献和实验
- 技术资料
- 库存:
现货
- 英文名:
Recombinant Human BDNF Protein, Flag Tag
- 保质期:
2年
- 供应商:
翌圣生物科技(上海)股份有限公司
- 保存条件:
-20度
- 规格:
100 μg(92129ES60)/500 μg(92129ES76)/25 μg(92129ES25)/1 mg(92129ES80)
| 规格: | 100 μg(92129ES60) | 产品价格: | ¥4245 |
|---|---|---|---|
| 规格: | 500 μg(92129ES76) | 产品价格: | ¥14857 |
| 规格: | 25 μg(92129ES25) | 产品价格: | ¥1285 |
| 规格: | 1 mg(92129ES80) | 产品价格: | ¥22285 |
产品描述:
BDNF(Brain-Derived Neurotrophic Factor,脑源性神经营养因子)属于神经营养因子(Neurotrophin)家族,该家族还包括NGF、NT-3和NT-4/5。是中枢神经系统中分布最广泛、研究最深入的神经营养因子。BDNF是一种在大脑神经元的生长、发育和存活中起关键作用的蛋白质。它主要在大脑的海马体、皮质和基底前脑区域产生。BDNF是大脑中一种重要的蛋白质,支持神经元的生长、存活和突触可塑性。在学习记忆、神经发育、神经退行性疾病以及精神疾病研究中是必不可少的工具蛋白。
产品属性:
| 产品别称 | BDNF, ANON2, BULN2, Abrineurin |
| 种属 | Mouse,Rat,Human |
| 表达序列 | His129-Arg247 |
| Accession | P23560-1 |
| 标签 | No Tag |
| 表达系统 | E.coli |
| 分子量 | The protein has a predicted MW of 13.51 kDa. The protein migrates to 15-19 kDa based on Tris-Bis PAGE result. |
| 纯度 | > 95% as determined by SDS-PAGE. |
| 内毒素 | < 1.0 EU per μg by the LAL method. |
| 制剂 | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 外观 | Lyophilized Powder |
| 生物活性 | ELISA Data:Immobilized Human/Murine/Rat BDNF, No Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human TrkB, His Tag with the EC50 of 0.24μg/ml determined by ELISA. |
| 复溶方法 | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
产品特色:
高纯度、高活性、低内毒素、高批间一致性
应用案例:
SDS-PAGE
Human/Mouse/Rat BDNF on SDS-PAGE under reduced condition. The purity is greater than 95%.
Bioactivity
Immobilized Human/Mouse/Rat BDNF, No Tag at 1 μg/mL (100 μl/well) on the plate. Dose response curve for Human TrkB, His Tag with the EC50 of 0.39 μg/ml determined by ELISA
储存条件:
-25 ~ -15℃保存,收到货之后有效期1年。
复溶后, -85 ~ -65℃保存,3个月有效期。
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文献和实验Strep/FLAG Tandem Affinity Purification (SF‐TAP) to Study Protein Interactions
a methodological workflow based on an SF?TAP tag comprising a doublet Strep?tag II and a FLAG moiety optimized for rapid as well as efficient tandem affinity purification of native proteins and protein complexes in higher eukaryotic cells. Depending
Production of Recombinant Neurotrophic Factors
factor (NGF), and its related protein family members, brainderived neurotrophic factor (BDNF or NT-2), and neurotrophin3 (NT.-3). The potential of producing mg and larger quantities of these agents will expedite our understanding of their functions
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