- ￥3500
- Tanda
- 7.81-500 ng/mL
- 中国
- TD711163
- 2025年11月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
800
- 供应商：
武汉天德
- 检测范围：
7.81-500 ng/mL
- 检测方法：
ELISA
- 应用：
用于人血清、血浆或其他相关生物液体中波形蛋白的测定。
- 适应物种：
Human
- 标记物：
FITC
- 样本：
Human
- 规格：
96Test
TD711163
人波形蛋白(VIM)ELISA试剂盒
| 英文名 | Human VIM(Vimentin) ELISA Kit | ||
| 别名 | VIM; FLJ36605 | ||
| 相关类别 | ELISA | 储存 | 冷藏（2-8℃） |
应用
用于人血清、血浆或其他相关生物液体中波形蛋白的测定。
For the determination of Human VIM(Vimentin) concentrations in serum, plasma and other related biological fluids.
属性
| 工作原理 | 本试剂盒采用的是双抗体夹心法酶联免疫吸附检测技术（ELISA）。测定样品中人波形蛋白水平。向预先包被了抗人波形蛋白抗体的酶标孔中，加入标准品和样本，温育后，加入生物素标记的抗波形蛋白抗体。再与HRP标记的链霉亲和素结合，形成免疫复合物，再经过温育和洗涤，去除未结合的酶，然后加入显色底物TMB，产生蓝色，并在酸的作用下转化成最终的黄色。最后，在450 nm处测定反应孔样品吸光度（OD）值，样本中的人波形蛋白浓度与OD值成正比，通过绘制标准曲线计算出样本中人波形蛋白的浓度。 |
| Purpose | This assay employs the Sandwich enzyme immunoassay technique. Determine the level of Human VIM(Vimentin) in the sample. Add standard and sample to the microplate that have been pre-coated with Anti-Human VIM(Vimentin) antibody. After incubation, add Biotin-conjugated anti-Human VIM(Vimentin) antibody. It is then combined with HRP-conjugated streptavidin to form an immune complex, then incubated and washed to remove unbound enzyme, and then added to the chromogenic substrate TMB to produce a blue color, and converted to the final yellow under the action of acid. Finally, the absorbance (OD) value was measured at 450 nm. The concentration of Human VIM(Vimentin) in the sample was proportional to the OD value. The concentration of Human VIM(Vimentin) in the sample can be calculated by drawing a standard curve. |
| 灵敏度 | 4.69 ng/mL |
| Sensitivity | 4.69 ng/mL |
| 检测范围 | 7.81-500 ng/mL |
| Examination range | 7.81-500 ng/mL |
| 特异性 | 可检测样本中的人波形蛋白，且与其类似物无明显交叉反应。 |
| Specificity | This kit recognizes Human VIM(Vimentin) in samples. No significant cross-reactivity or interference between Human VIM(Vimentin) and analogues was ob/served. |
| 重复性 | 三种不同浓度的样本在同一块板子上分别检测20次，以及在不同的板子上分别检测20次，板内和板间变异系数均小于10%。 |
| Repeatability | Three different concentrations of samples were detected 20 times on the same board and 20 times on different boards. Within the plate or between the plates, the coefficient of variation is <10%. |
| 回收率 | 分别往稀释不同倍数的5个样本中加入已知浓度的目标蛋白，做回收实验，得出回收率范围和平均回收率。 |
| Recovery | A known concentration of the target protein was added to 5 samples which were diluted to different multiples, and a recovery experiment was performed to obtain a recovery range and an average recovery rate. |
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文献和实验Discovery of Lamin B1 and Vimentin as Circulating Biomarkers for Early Hepatocellular Carcinoma
electrophoresis (2DE) and MALDI-TOF/TOF mass spectrometry, our groups have identified lamin B1 (LMNB1) and vimentin (VIM) as promising biomarkers for detection of early HCC. Protein levels of both biomarkers were significantly elevated in cancerous tissues
所发生的肿瘤，是肿瘤病理学中最棘手和最疑难的问题之一。随着免疫组化技术在肿瘤鉴别诊断中的应用，已经克服了许多以往无法克服或者难以克服的问题。当前，应用于这方面检测的抗体越来越多，下面将介绍几种常用的抗体： 1.波形蛋白(Vimentin ,Vim) Vim是原始的中间丝，它存在于所有的间叶组织细胞中，可以向神经胶质，肌源性细胞和上皮细胞中间丝演变。Vim是一种单克隆抗体，临床应用来作鉴别诊断的有：鉴别癌与内癌，前者阴性，后者阳性;鉴别小细胞性肿瘤，这时应多用几种抗体，如CK
Western时，您会得到一个量化值，哪些组分占多少。Sampler Kit包含细胞质MEK1/2、线粒体凋亡诱导因子(AIF)、组蛋白H3和细胞骨架波形蛋白的兔单克隆抗体，以及山羊抗兔IgG。 研究人员也可利用试剂盒中的建议，或搜索文献，组装自己的Western blot或ELISA试剂盒。不过Cormier也提醒，并非每个抗体都有着它应有的特异性。 下游的分析 与超速离心相比，基于试剂盒的细胞分级分离方法快速又方便，也适合多个下游应用，如EMSA、报告基因分析、酶活
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