This assay employs the Sandwich enzyme immunoassay technique. Determine the level of Human PCSK9 in the sample. Add standard and sample to the microplate that have been pre-coated with Anti-Human PCSK9 antibody. After incubation, add Biotin-conjugated anti-Human PCSK9 antibody. It is then combined with HRP-conjugated streptavidin to form an immune complex, then incubated and washed to remove unbound enzyme, and then added to the chromogenic substrate TMB to produce a blue color, and converted to the final yellow under the action of acid. Finally, the absorbance (OD) value was measured at 450 nm. The concentration of Human PCSK9 in the sample was proportional to the OD value. The concentration of Human PCSK9 in the sample can be calculated by drawing a standard curve.
灵敏度
46.88 pg/mL
Sensitivity
46.88 pg/mL
检测范围
78.13-5000 pg/mL
Examination range
78.13-5000 pg/mL
特异性
可检测样本中的人前蛋白转化酶枯草溶菌素9,且与其类似物无明显交叉反应。
Specificity
This kit recognizes Human PCSK9 in samples. No significant cross-reactivity or interference between Human ApoB(Apolipoprotein B) and analogues was ob/served.Human PCSK9 in samples. No significant cross-reactivity or interference between Human PCSK9 and analogues was ob/served.
Three different concentrations of samples were detected 20 times on the same board and 20 times on different boards. Within the plate or between the plates, the coefficient of variation is <10%.
回收率
分别往稀释不同倍数的5个样本中加入已知浓度的目标蛋白,做回收实验,得出回收率范围和平均回收率。
Recovery
A known concentration of the target protein was added to 5 samples which were diluted to different multiples, and a recovery experiment was performed to obtain a recovery range and an average recovery rate.