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文献和实验If titre is positive do one of the following 2 weeks later: Boost tail vein with 20 ug- 50 ug of Antigen in PBS and proceed with fusion on day 4. OR Boost subcutaneously with 50 ug - 100 ug of Antigen in PBS and proceed
Radiolabeling of Monoclonal Antibodies
Radiolabeling of monoclonal antibodies (mAbs) is often one of the principal methods for the in vitro and in vivo assessment of these reagents, whether this is determining the affinity of a new reagent, preparing for preclinical testing
Polyclonal and Monoclonal Antibodies
the serum. 4) Serum can be stored at -20 C after adding Glycerol to 50%. If monoclonal antibodies are desired, once you have a "good" polyclonal and a reliable screening method proceed to the next step. Immunization of Rabbits. Rabbits
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