BioDynami开发NGS DNA建库试剂盒(Ion Torrent平台),用于利用Ion Torrent平台构建高质量的DNA文库用于二代测序。该试剂盒使用短的双链DNA片段(钝端和/或粘端)作为NGS文库构建的输入DNA,与酶促法(BioDynami DNA Fragmentation Enzyme Mix和DNA Fragmentation & a -tail Enzyme Mix等)和机械法(超声、雾化等)产生的DNA片段兼容。BioDynami独特的技术提高了库转换效率,并消除了插入串联连接。库复用可多达12个样本。
The NGS DNA Library Prep Kit (Ion Torrent platform) was developed for construction of high quality DNA libraries for next generation sequencing using Ion Torrent platform. The kit uses short double strand DNA fragments (blunt ends and/or sticky ends) as input DNA for NGS library construction, and is compatible with DNA fragments generated from both enzymatic methods (BioDynami DNA Fragmentation Enzyme Mix and DNA Fragmentation & A-tailing Enzyme Mix etc) and mechanical methods (sonication, nebulization etc.). Our unique technology increases library conversion efficiency and eliminates insert concatemer ligation. Library multiplexing up to 12 samples is possible.
NGS DNA建库试剂盒工作流程
产品特性:
★ 操作简单:三个步骤的反应都在一个试管里;
★ 快速的实验方案;
★ 与其他试剂盒相比,保证了较高的库转换效率;
★ 总操作时间约为1小时;
★ 操作时间只有大约5分钟;
★ 输入DNA量:从50ng到1ug;
★ 无插入串联连接。
Kit features
Simple procedure: Reactions for all three steps are in one tube
Fast protocol
Total protocol time is around 1 hour
Hands-on time is only ~5 minutes
Guaranteed high library conversion efficiency as compared to other kits