Product DescriptionHepatitis B virus (HBV) is a human pathogen, causing serious liver disease. The HBV surface protein antigens (HBsAg) are comprised of three carboxyl co terminal HBs proteins termed large (LHBs), middle (MHBs) and small (SHBs) protein. LHBs & MHBs also share the highly hydrophobic, repetitive, membrane spanning S domain. In addition, LHBs has a 119 amino acid region called preS1.Product Properties
Synonyms
L glycoprotein,L-HBsAg,Large S protein,Large surface protein,Major surface antigen.
Accession
Q1PD03
GeneID
10407
Source
E.coli-derived HBsAg-preS1
Molecular Weight
Approximately 12.6 kDa, a single non-glycosylated polypeptide chain containing 119 amino acids,with an N-terminal Met
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
> 97% by SDS-PAGE and HPLC analyses.
Endotoxin
< 1.0 EU per 1μg of the protein by the LAL method.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 50 mM NaCl.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Shipping and StorageThe products are shipped with ice pack and can be stored at -20℃ for 1 year.1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 °C under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first usedand avoid repeated freeze-thaw cycles. Cautions1.For your safety and health, please wear lab coats and disposable gloves for operation. 2.For research use only!