产品简介:
BioDynami开发了基于illumina平台的NGS 古DNA建库试剂盒,以50 ng - 500 ng的古DNA作为输入构建高质量的文库。该试剂盒基于单链DNA文库制备,对古DNA样本更有效。高降解的古DNA在建库过程中存在一定难度,使用常规建库方法往往导致建库转化率较低。科学家们已经证明,使用ssDNA文库准备方法进行古DNA文库建库的效果显著优于使用dsDNA文库建库方法。
The NGS Ancient DNA Library Prep Kit (illumina platform) was developed for construction of high quality libraries using ancient DNA (50 ng – 500 ng) as input. The kit is based on single stranded DNA library preparation, which is more effective for ancient DNA samples. Highly degraded ancient DNA is difficult for the library preparation and usually result in low library conversion rate using regular library prep approach. It has been demonstrated by scientists that ancient DNA library prep using ssDNA library prep approach has significant better outcome than that of dsDNA library prep approach.
BioDynami NGS 古DNA建库试剂盒工作流程
试剂盒有三种index类型:
Non-index(Cat.# 30084):没有索引。
Index(Cat.# 30085):BioDynami的每个索引引物包含6个碱基的唯一序列。通过48个样本,可实现古DNA文库的复用。
Unique dual index(Cat.# 30086):通过BioDynami独特的双索引,可以对多达96个样本进行多路复用。在BioDynami独特的Four-Base Difference Index System中,每个索引至少有4个碱基不同于其他8碱基索引。独特的双索引引物消除了测序的错误,如解复用错误、索引跳跃和读取错误赋值等。独特的双索引引物是96孔板格式96预混唯一对i5和i7索引引物。
Three index types are available for the kit:
Non-index (Cat.# 30084): Libraries do not have index.
Index (Cat.# 30085): Each of our index primers contains a unique barcode sequence with 6 bases. Multiplexing of the ancient DNA library is possible with 48 samples.
Unique dual index (Cat.# 30086): The capacity for multiplexing of ancient DNA library is up to 96 samples with our unique dual indexes. With BioDynami’s unique Four-Base Difference Index System, each of the index has at least 4 bases different from others in the 8-base index. The unique dual indexing primers remove errors of sequencing such as de-multiplexing errors, index hopping, and reads mis-assignment etc. The unique dual index primers is in a 96-well plate format with 96 pre-mixed unique pairs of i5 and i7 index primers.
试剂盒优势:
★ 动手时间:10分钟
★ 总时间:1.5小时
★ 迅速
★ 工作流程简单
★ 所需磁珠量:50%以下
★ 保证质量:高库转换效率
★ 古DNA输入:50 - 500 ng
Kit advantages
- Fast
- Hands-on time: 10 minutes
- Total time: 1.5 hours
- Simple workflow
- Amount of magnetic beads needed: Less than 50%
- Guaranteed quality: High library conversion efficiency
- Ancient DNA input: From 50 ng to 500 ng
产品订购:
产品名称 |
货号 |
产品规格 |
NGS Ancient DNA Library Prep Kit |
30084S |
non-index,24 reactions |
NGS Ancient DNA Library Prep Kit |
30084L |
non-index,48 reactions |
NGS Ancient DNA Library Prep Kit |
30085S |
index,24 reactions |
NGS Ancient DNA Library Prep Kit |
30085L |
index,48 reactions |
NGS Ancient DNA Library Prep Kit |
30086S |
unique dual index,96 reactions |
NGS Ancient DNA Library Prep Kit |
30086L |
unique dual index,192 reactions |