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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Infectious bronchitis virus Spike glycoprotein S1 subunit (S1), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100μg/20μg
| 规格: | 1mg | 产品价格: | ¥14796.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥4374.0 |
| 规格: | 20μg | 产品价格: | ¥2328.0 |
纯度:
Greater than 90% as determined by SDS-PAGE.基因名:
S1Uniprot No.:
D9IAI3别名:
/种属:
Infectious bronchitis virus蛋白长度:
Partial来源:
E.coli分子量:
49.2 kDa表达区域:
231-539aa氨基酸序列:
ACQYNTGNFSDGFYPFTNVSLVKERFVVYRETSVNTTLVLTNFTFTNVSNASPNTGGVNTINIYQTQTAQSGYYNFNFSFLSSFVYKQSDFMYGSYHPKCDFRPETINNGLWFNSLSVSLAYGPLQGGCKQSVFSNRATCCYAYSYNGPRLCKGVYIGELPQYFECGLLVYVIKSDGSRIQTRNEPLVLTHYNYNNITLDRCVEYNIYGRSGQGFIINVTASAANYNYLADGGLAILDTSGAIDIFVVQGEYGPNYYKVNPCEDVNQQFVVSGGGIVGVLTSHNETGSQQLENRFYVKLTNSTRRTRRL蛋白标签:
N-terminal 6xHis-B2M-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
/内毒素:
Not test.SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.LC-MS Image Description/Western Blot:
/Product types:
Developed ProteinBiological_Activity:
/Research Areas:
OthersReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
"Characterization of a live attenuated infectious bronchitis virus vaccine candidate derived from the Israeli isolate IS/1494/06." Meir R., Simanov L. Submitted (APR-2010)Function:
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文献和实验and feline infectious peritonitis virus. Key to the two-step method is the efficient selection of recombinant viruses based on host cell switching. The first step consists of the preparation—using this selection principle—of an interspecies chimeric
[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
factor 4A, isoform 2 (EIF4A2), mRNA 2935 NM_001863 Homo sapiens cytochrome c oxidase subunit VIb (COX6B ), nuclear gene encoding mitochondrial protein, mRNA 1719 NM_001997 Homo sapiens Finkel-Biskis-Reilly murine sarcoma virus (FBR-MuSV
包埋组织中提取DNA的基本步骤,是在常规DNA提取方法的基础上改良和演变而来。Goelz等(1985)最先提出的石蜡包埋组织DNA提取技术,是用机械的方法破碎组织,切除多余的石蜡,直接进入含SDS和高浓度蛋白酶K(1mg/ml)的提取缓冲液加温孵育,然后进行苯酚和氯仿抽提。所得DNA虽不完整,但可被限制性内切酶切割,因而适于点杂交,印迹杂交等多种分子生物学实验。Dubeau等(1986)在上述方法上作了改进。首先通过组织切片用二甲苯脱蜡,保证了组织的完全破碎,使细胞与消化液充分接触,使DNA释放和蛋白
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