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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Mesocricetus auratus Beta-2-microglobulin (B2m)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100μg/20μg
| 规格: | 1mg | 产品价格: | ¥11868.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥3510.0 |
| 规格: | 20μg | 产品价格: | ¥2340.0 |
纯度:
Greater than 90% as determined by SDS-PAGE.基因名:
B2mUniprot No.:
A0A1U7QLF7别名:
/种属:
Mesocricetus auratus (Golden hamster)蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
11.9 kDa表达区域:
21-119aa氨基酸序列:
IQRPPQVQVYTRHPPEDGKPNFLNCYVSQFHPPHIEIELLKNGEKMDKVELSDLSFNKDWSFYLLAHREFVPTATDKYACRVSHITLKEPKVVTWERDM蛋白标签:
Tag-Free产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
/内毒素:
Not test.SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.LC-MS Image Description/Western Blot:
/Product types:
Developed ProteinBiological_Activity:
/Research Areas:
OthersReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
1 RefSeq Submitted (APR-2022) to UniProtKB Cited for: IDENTIFICATION.Function:
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文献和实验实验,是否在你的不同实验组中是稳定表达的呢? 我们先一起来了解下面这个案例: 该实验主要目的: NIH3T3 细胞系在无血清培养基中培养 24 h 后,添加 15% 血清,观察 8 h 内特定基因的表达量。本实验选取了两个内参基因:GAPDH 与β-2-microglobulin进行实验验证。 在不同实验组下,通过利用 2-△CT(△CT= CT,Time x - CT,Time 0)计算得到 9 个数据,通过进行统计学分析,判定这 9 个数据差异是否显著。实验结果表明,GAPDH p<
下调不能说明活性降低,因为决定GSK3-beta的活性的还与其磷酸化的比例和磷酸化的位点有关。所以你除了要做GSK3-beta总的蛋白表达之外,还要做磷酸化的GSK3-beta的蛋白表达。 (2)应该活性形式和非活性形式都做。一般来讲,GSK3-beta在Ser9位点磷酸化之后活性收到抑制,而在216位点磷酸化之后,其活性收到加强。因此建议将GSK3-beta的两个磷酸化位点都做了,另外还要同时检测GSK3-beta的总蛋白表达,这样才能全面的说明问题。
% polyacrylamide gel containing blue-stained beta-casein (0.5 mg/ml)(Novex). After electrophoresis was complete, the gel was incubated for 1 hour at 25℃ in a 2.5% Triton X-100 solution, washed two times, 20 minutes each, with water and then incubated overnight
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