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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Mouse Palmitoyl-protein thioesterase 1 (Ppt1)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100μg/20μg
| 规格: | 1mg | 产品价格: | ¥26400.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥3108.0 |
| 规格: | 20μg | 产品价格: | ¥1908.0 |
纯度:
Greater than 90% as determined by SDS-PAGE.基因名:
Ppt1Uniprot No.:
O88531别名:
(PPT-1)(Palmitoyl-protein hydrolase 1)种属:
Mus musculus (Mouse)蛋白长度:
Full Length of Mature Protein来源:
Mammalian cell分子量:
33.0 kDa表达区域:
28-306aa氨基酸序列:
DPPSPPPLVIWHGMGDSCCNPMSMGVIKKMVEKEIPGIYVLSLEIGKNMMEDVENSFFLNVNVQVNMVCQILEKDPKLQQGYNAIGFSQGGQFLRAVAQRCPTPPMMTLISVGGQHQGVFGLPRCPGESSHICDFIRKSLNAGAYSKLVQERLVQAQYWHDPIKESVYRNYSIFLADINQERCVNESYKKNLMALKKFVMVKFFNDSIVDPVDSEWFGFYRSGQAKETIPLQESTLYTEDRLGLKKMDKAGKLVFLAKEGDHLQISKEWFTAHIIPFLK蛋白标签:
C-terminal 10xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
Removes thioester-linked fatty acyl groups such as palmitate from modified cysteine residues in proteins or peptides during lysosomal degradation. Prefers acyl chain lengths of 14 to 18 carbons.内毒素:
Not test.SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.LC-MS Image Description/Western Blot:
/Product types:
In Stock ProteinBiological_Activity:
/Research Areas:
OthersReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
"Behavioral assessment in mouse models of neuronal ceroid lipofuscinosis using a light-cued T-maze." Wendt K.D., Lei B., Schachtman T.R., Tullis G.E., Ibe M.E., Katz M.L. Behav Brain Res 161:175-182(2005)Function:
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文献和实验层析复性蛋白质 李明1 苏志国1 Janson Jan-Christer2 (1 生化工程国家重点实验室,中国科学院过程工程研究所,北京,100080 2 表面生物技术系,Uppsala 大学,Uppsala, 瑞典) 摘要在生物学和生物医学中,尤其在制药行业中,蛋白质的体外折叠复性是一个比较棘手的问题。最近利用层析方法复性成为研究的热点,本综述主要介绍层析复性方法的进展。 随着基因工程和生物技术的发展,蛋白质克隆与表达已经趋于成熟。在重组蛋白质生产的过程中,目标产物的异源
. et al. (1998) Oral Microbiol. Immunol. 13, 166–172 59. Monitoring of recombinant protein production using bioluminescence in a semiautomated fermentation process.Trezzani, I. et al. (2003) Biotechnol. Prog. 19, 1377–1382 60. Kinetic analysis
V294.PART II,Chapter 4 细胞迁移实验之Dunn趋化迁移培养皿法及时序显微镜分析法
). 4. In addition to studying the response of BMMs isolated from knock-out mice, theDCC can be used to characterize the chemotactic response of cells expressingtagged proteins introduced either by microinjection of recombinant protein ordeoxyribonucleic acid (16
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