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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Alpha-2A adrenergic receptor (ADRA2A)-VLPs
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human Alpha-2A adrenergic receptor (ADRA2A)-VLPs品名简称:
Recombinant Human ADRA2A protein-VLPs货号:
CSB-MP001388HU规格:
20μg价格:
3720LC-MS/Western Blot:
/SDS-PAGE:
CSB-MP001388HU is detected by Mouse anti-6*His monoclonal antibody.纯度:
The purity information is not available for VLPs proteins.内毒素:
Not test生物活性:
/基因名:
ADRA2AUniprot No.:
P08913别名:
(Alpha-2 adrenergic receptor subtype C10)(Alpha-2A adrenoreceptor)(Alpha-2A adrenoceptor)(Alpha-2AAR)种属:
Homo sapiens (Human)蛋白长度:
Full Length来源:
Mammalian cell分子量:
52.0 kDa表达区域:
1-465aa氨基酸序列:
MFRQEQPLAEGSFAPMGSLQPDAGNASWNGTEAPGGGARATPYSLQVTLTLVCLAGLLMLLTVFGNVLVIIAVFTSRALKAPQNLFLVSLASADILVATLVIPFSLANEVMGYWYFGKAWCEIYLALDVLFCTSSIVHLCAISLDRYWSITQAIEYNLKRTPRRIKAIIITVWVISAVISFPPLISIEKKGGGGGPQPAEPRCEINDQKWYVISSCIGSFFAPCLIMILVYVRIYQIAKRRTRVPPSRRGPDAVAAPPGGTERRPNGLGPERSAGPGGAEAEPLPTQLNGAPGEPAPAGPRDTDALDLEESSSSDHAERPPGPRRPERGPRGKGKARASQVKPGDSLPRRGPGATGIGTPAAGPGEERVGAAKASRWRGRQNREKRFTFVLAVVIGVFVVCWFPFFFTYTLTAVGCSVPRTLFKFFFWFGYCNSSLNPVIYTIFNHDFRRAFKKILCRGDRKRIV蛋白标签:
C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions)产品提供形式:
Lyophilized powder缓冲液:
Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein indeionized sterile water to a concentration of 0.1-1.0 mg/mL.Aliquot for long-term storage at -80℃. Solubilize for 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
The VLPs are expressed from human 293 cells (HEK293).Mix the sample gently by repeatedly pipetting it up and down. Do not vortex.Repeated freezing and thawing is not recommended.Store the protein at -20℃/-80℃ upon receiving it, and ensure to avoid repeated freezing and thawing, otherwise, it will affect the protein activity. The immunization strategy should be optimized (antigen dose, regimen and adjuvant).功能1:
Alpha-2 adrenergic receptors mediate the catecholamine-induced inhibition of adenylate cyclase through the action of G proteins. The rank order of potency for agonists of this receptor is oxymetazoline > clonidine > epinephrine > norepinephrine > phenylephrine > dopamine > p-synephrine > p-tyramine > serotonin = p-octopamine. For antagonists, the rank order is yohimbine > phentolamine = mianserine > chlorpromazine = spiperone = prazosin > propanolol > alprenolol = pindolol.功能2:
/文献:
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004)研究领域:
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文献和实验Analyses of Adrenergic Receptor Sequences
with known adrenergic receptor sequences in order to verify the receptor and subtype, to submit the sequence to GenBank, and to analyze the predicted secondary structure of the receptor.
Isolation of Adrenergic Receptor Genes
In order to isolate a single gene, phage or cosmid libraries can be screened by the conventional technique of hybridization as described by Sambrook et al. (1 ) using end-labeled oligonucleotide probes or gene-specific probes. The probes
In Situ Hybridization of Adrenergic Receptor mRNA in Brain
, and temperature control; these findings have renewed interest in examining human CNS tissue for expression of adrenergic receptor subtype mRNA in specific brain regions. In situ hybridization enables detection of rare mRNA species in specific cells
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