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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Alpha-2A adrenergic receptor (ADRA2A)-VLPs
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100μg/20μg
| 规格: | 1mg | 产品价格: | ¥40068.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥7560.0 |
| 规格: | 20μg | 产品价格: | ¥3780.0 |
纯度:
The purity information is not available for VLPs proteins.基因名:
ADRA2AUniprot No.:
P08913别名:
(Alpha-2 adrenergic receptor subtype C10)(Alpha-2A adrenoreceptor)(Alpha-2A adrenoceptor)(Alpha-2AAR)种属:
Homo sapiens (Human)蛋白长度:
Full Length来源:
Mammalian cell分子量:
52.0 kDa表达区域:
1-465aa氨基酸序列:
MFRQEQPLAEGSFAPMGSLQPDAGNASWNGTEAPGGGARATPYSLQVTLTLVCLAGLLMLLTVFGNVLVIIAVFTSRALKAPQNLFLVSLASADILVATLVIPFSLANEVMGYWYFGKAWCEIYLALDVLFCTSSIVHLCAISLDRYWSITQAIEYNLKRTPRRIKAIIITVWVISAVISFPPLISIEKKGGGGGPQPAEPRCEINDQKWYVISSCIGSFFAPCLIMILVYVRIYQIAKRRTRVPPSRRGPDAVAAPPGGTERRPNGLGPERSAGPGGAEAEPLPTQLNGAPGEPAPAGPRDTDALDLEESSSSDHAERPPGPRRPERGPRGKGKARASQVKPGDSLPRRGPGATGIGTPAAGPGEERVGAAKASRWRGRQNREKRFTFVLAVVIGVFVVCWFPFFFTYTLTAVGCSVPRTLFKFFFWFGYCNSSLNPVIYTIFNHDFRRAFKKILCRGDRKRIV蛋白标签:
C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions)产品提供形式:
Lyophilized powder缓冲液:
Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4货期:
3-7 business days注意事项:
The VLPs are expressed from human 293 cells (HEK293).Mix the sample gently by repeatedly pipetting it up and down. Do not vortex.Repeated freezing and thawing is not recommended.Store the protein at -20℃/-80℃ upon receiving it, and ensure to avoid repeated freezing and thawing, otherwise, it will affect the protein activity. The immunization strategy should be optimized (antigen dose, regimen and adjuvant).功能:
Alpha-2 adrenergic receptors mediate the catecholamine-induced inhibition of adenylate cyclase through the action of G proteins. The rank order of potency for agonists of this receptor is oxymetazoline > clonidine > epinephrine > norepinephrine > phenylephrine > dopamine > p-synephrine > p-tyramine > serotonin = p-octopamine. For antagonists, the rank order is yohimbine > phentolamine = mianserine > chlorpromazine = spiperone = prazosin > propanolol > alprenolol = pindolol.内毒素:
Not test.SDS-PAGE:
CSB-MP001388HU is detected by Mouse anti-6*His monoclonal antibody.LC-MS Image Description/Western Blot:
/Product types:
MP-VLP Transmembrane Protein & In Stock ProteinBiological_Activity:
/Research Areas:
NeuroscienceReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein indeionized sterile water to a concentration of 0.1-1.0 mg/mL.Aliquot for long-term storage at -80℃. Solubilize for 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.Reference:
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004)Function:
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文献和实验Distribution of 2A- and 2C-Adrenergic Receptor Immunoreactivity in the Central Nervous System
in the regulation of analgesia, memory, and central cardiovascular control (1 ). Three subtypes of the receptor have been identified (α2A -, α2B -, and α2C -ARs; for review, see ref. 2 ). Comparisons of the three subtypes show a high degree of sequence similarity
In Situ Hybridization of Adrenergic Receptor mRNA in Brain
, and temperature control; these findings have renewed interest in examining human CNS tissue for expression of adrenergic receptor subtype mRNA in specific brain regions. In situ hybridization enables detection of rare mRNA species in specific cells
For quite some time, the majority of GPCR models have been based on a single template structure: dark-adapted bovine rhodopsin. The recent solution of β2AR, β1AR and adenosine A2A receptor crystal structures has dramatically expanded the GPCR
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