IL2RA/IL2RA蛋白Recombinant Human

纯度：

Greater than 95% as determined by SDS-PAGE.

基因名：

IL2RA

Uniprot No.：

P01589

别名：

IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA; TAC antigen; p55; CD25

种属：

Homo sapiens (Human)

蛋白长度：

Partial

来源：

Mammalian cell

分子量：

23.6 kDa

表达区域：

22-213aa

氨基酸序列：

ELCDDDPPEIPHATFKAMAYKEGTMLNCECKRGFRRIKSGSLYMLCTGNSSHSSWDNQCQCTSSATRNTTKQVTPQPEEQKERKTTEMQSPMQPVDQASLPGHCREPPPWENEATERIYHFVVGQMVYYQCVQGYRALHRGPAESVCKMTHGKTRWTQPQLICTGEMETSQFPGEEKPQASPEGRPESETSC

蛋白标签：

C-terminal 10xHis-tagged

产品提供形式：

Lyophilized powder

缓冲液：

Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0

货期：

3-7 business days

注意事项：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

功能：

Involve in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity.

内毒素：

Less than 1.0 EU/ug as determined by LAL method.

SDS-PAGE:

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

LC-MS Image Description/Western Blot:

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Product types:

Active Protein & In Stock Protein

Biological_Activity:

①Measured by its binding ability in a functional ELISA. Immobilized Human IL2RA at 2 μg/mL can bind Anti-IL2RA recombinant antibody (CSB-RA011649MA1HU) , the EC50 is 2.463-3.353 ng/mL.②Measured by its binding ability in a functional ELISA. Immobilized Human IL2RA at 2 μg/mL can bind Human IL2(CSB-MP011629HU) , the EC50 is 1.693-2.039 ng/mL.

Research Areas:

Cancer

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Reference:

"Molecular cloning of cDNA encoding human interleukin-2 receptor." Nikaido T., Shimizu A., Ishida N., Sabe H., Teshigawara K., Maeda M., Uchiyama T., Yodoi J., Honjo T. Nature 311:631-635 (1984)

Function:

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