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- 文献和实验
- 技术资料
- 保存条件:
-20℃/-80℃
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Bovine Butyrophilin subfamily 1 member A1 (BTN1A1), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100ug/20ug
| 规格: | 1mg | 产品价格: | ¥12372.0 |
|---|---|---|---|
| 规格: | 100ug | 产品价格: | ¥2880.0 |
| 规格: | 20ug | 产品价格: | ¥1536.0 |
Research Areas
OthersUniprot ID
P18892Gene Names
BTN1A1Alternative Name(s)
(BT)Lead Time
3-7 business daysOrganism
Bos taurus (Bovine)Source
E.coliExpression Region
27-242aaProtein Length
PartialTag Info
N-terminal 10xHis-tagged and C-terminal Myc-taggedTarget Protein Sequence
APFDVIGPQEPILAVVGEDAELPCRLSPNVSAKGMELRWFREKVSPAVFVSREGQEQEGEEMAEYRGRVSLVEDHIAEGSVAVRIQEVKASDDGEYRCFFRQDENYEEAIVHLKVAALGSDPHISMKVQESGEIQLECTSVGWYPEPQVQWRTHRGEEFPSMSESRNPDEEGLFTVRASVIIRDSSMKNVSCCIRNLLLGQEKEVEVSIPASFFPRMW
31.8 kDaPurity
Greater than 85% as determined by SDS-PAGE.Endotoxin
Not test.Biological_Activity
/Form
Liquid or Lyophilized powderBuffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.Relevance
May function in the secretion of milk-fat droplets. May act as a specific membrane-associated receptor for the association of cytoplasmic droplets with the apical plasma membrane. Inhibits the proliferation of CD4 and CD8 T-cells activated by anti-CD3 antibodies, T-cell metabolism and IL2 and IFNG secretion.Function
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-100mM 2-BME或DTT,也有文献使用5mM浓度。在较粗放的条件下,可以使用5ml/l的浓度。还原剂的使用浓度与蛋白二硫键的数目无关,而有些没有二硫键的蛋白加不加还原剂无影响,如牛生长激素包涵体的增溶。对于目标蛋白没有二硫键某些包涵体的增溶,有时还原剂的使用也是必要的,可能由于含二硫键的杂蛋白影响了包涵体的溶解。[4] 二、复性: 由于包涵体中的重组蛋白缺乏生物学活性,加上剧烈的处理条件,使蛋白的高级结构破坏,因此重组蛋白的复性特别必要。 通过缓慢去除变性剂使目标蛋白从变性
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that Drives Pancreatic Cancer Metastasis,Cancer Res,2026 Oct 02 图源:[6] 北京协和医学院牛亚梅/梁智勇研究团队在Cancer Research发表了一篇研究,揭示癌相关成纤维细胞(CAF)中m6A去甲基化酶ALKBH5通过去除HSF1 mRNA上m6A修饰,解除IGF2BP3介导的翻译抑制,促进HSF1蛋白合成。HSF1通过激活LIF基因远端增强子促进促肿瘤因子LIF分泌,建立ALKBH5-HSF1-LIF旁分泌信号轴,增强
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