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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Serpin B10 (SERPINB10)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human Serpin B10 (SERPINB10)品名简称:
Recombinant Human SERPINB10 protein货号:
CSB-YP021066HUa4规格:
20μg价格:
2208LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
SERPINB10Uniprot No.:
P48595别名:
(Bomapin)(Peptidase inhibitor 10)(PI-10)种属:
Homo sapiens (Human)蛋白长度:
Full Length来源:
Yeast分子量:
58.5 kDa表达区域:
1-397aa氨基酸序列:
MDSLATSINQFALELSKKLAESAQGKNIFFSSWSISTSLTIVYLGAKGTTAAQMAQVLQFNRDQGVKCDPESEKKRKMEFNLSNSEEIHSDFQTLISEILKPNDDYLLKTANAIYGEKTYAFHNKYLEDMKTYFGAEPQPVNFVEASDQIRKDINSWVERQTEGKIQNLLPDDSVDSTTRMILVNALYFKGIWEHQFLVQNTTEKPFRINETTSKPVQMMFMKKKLHIFHIEKPKAVGLQLYYKSRDLSLLILLPEDINGLEQLEKAITYEKLNEWTSADMMELYEVQLHLPKFKLEDSYDLKSTLSSMGMSDAFSQSKADFSGMSSARNLFLSNVFHKAFVEINEQGTEAAAGSGSEIDIRIRVPSIEFNANHPFLFFIRHNKTNTILFYGRLCSP蛋白标签:
N-terminal 6xHis-sumostar-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
25-35 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Protease inhibitor that may play a role in the regulation of protease activities during hematopoiesis and apoptosis induced by TNF. May regulate protease activities in the cytoplasm and in the nucleus.功能2:
/文献:
"Proteome profiling of human neutrophil granule subsets, secretory vesicles, and cell membrane: correlation with transcriptome profiling of neutrophil precursors." Rorvig S., Ostergaard O., Heegaard N.H., Borregaard N. J Leukoc Biol 94:711-721(2013)研究领域:
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文献和实验来自于 HIV 病人,在这种特殊的情况下,我们希望能够对样本做固定处理或者是预固定处理,这样会大大增加操作人员的安全性。对蛋白翻译后修饰的检测在蛋白翻译后修饰(磷酸化、乙酰化、甲基化等)的研究过程中,固定剂不仅可以有效的「固定」住翻译后修饰的位点(丝氨酸、苏氨酸、酪氨酸等),也可以防止目标位点在活细胞中很快降解(比如磷酸酶会去除磷酸化修饰、去甲基化酶会移除甲基化修饰等)。下面的例子是用 IL- 6 的重组蛋白刺激人外周血 15 分钟后看 STAT3 第 705 位酪氨酸的磷酸化水平,在刺激完样本后就需要
案例精选: 2021 年 7 月,复旦大学附属中山医院肺重 症医学科在 Respiratory Research 上发是 表题为:SERPINB10 contributes to asthma by inbibiting the apoptosis of allergenic Th2 cells 的研究论文。 该研究表明,辅助型 T 细胞 2 (Th2) 及其分 泌的细胞因子与哮喘发病机制密切相关, 丝氨酸蛋白抑制剂家族成员 SERPINB10 与 哮喘的过敏性炎症有关,本研究,作者通
因子或重组蛋白溶液加入到培养体系内即可。 其实,说明书上推荐的浓度对于一般的实验来讲是比较高的。所以用户通常会将该溶液进一步稀释后再放在4℃保存,待1周内用完。如进行稀释,必须遵照下面第4步的方法,即需用含载体蛋白的溶液进行稀释,否则稀释后的细胞因子或重组蛋白很容易会粘附在管壁或瓶壁上,使得溶液中的细胞因子或重组蛋白的浓度下降,细胞因子或重组蛋白的总活性则大为减弱。 第4步:如要长期保存,则需用含载体蛋白(如0.1% BSA,或10% FBS,或5% HSA)的溶液进一步稀释,然后分装冻存
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