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- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Rat Aquaporin-2 (Aqp2)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Rat Aquaporin-2 (Aqp2)品名简称:
Recombinant Rat Aqp2 protein货号:
CSB-CF001962RA规格:
20μg价格:
9720LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
Aqp2Uniprot No.:
P34080别名:
(AQP-2)(ADH water channel)(Aquaporin-CD)(AQP-CD)(Collecting duct water channel protein)(WCH-CD)(Water channel protein for renal collecting duct)种属:
Rattus norvegicus (Rat)蛋白长度:
Full Length来源:
in vitro E.coli expression system分子量:
30.4 kDa表达区域:
1-271aa氨基酸序列:
MWELRSIAFSRAVLAEFLATLLFVFFGLGSALQWASSPPSVLQIAVAFGLGIGILVQALGHVSGAHINPAVTVACLVGCHVSFLRAAFYVAAQLLGAVAGAAILHEITPVEIRGDLAVNALHNNATAGQAVTVELFLTMQLVLCIFASTDERRGDNLGSPALSIGFSVTLGHLLGIYFTGCSMNPARSLAPAVVTGKFDDHWVFWIGPLVGAIIGSLLYNYLLFPSAKSLQERLAVLKGLEPDTDWEEREVRRRQSVELHSPQSLPRGSKA蛋白标签:
N-terminal 10xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient . Plays an essential role in renal water homeostasis .功能2:
/文献:
"Expression, functional analysis, and in situ hybridization of a cloned rat kidney collecting duct water channel." Ma T., Hasegawa H., Skach W., Frigeri A., Verkman A.S. Am. J. Physiol. 266:C189-C197(1994)研究领域:
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文献和实验Secretory Vesicle Swelling by Atomic Force Microscopy
secretory vesicles in exocrine pancreas, swell in response to GTP mediated by a G�i3 protein. Subsequently, our studies demonstrated that the water channel aquaporin-1 (AQP1) is also present at the zymogen granule membrane and participates in rapid GTP
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
cytometric analysis, the OP9-DL1 will fluoresce in the FITC (FL1) channel. These cells can be gated-out using forward and side-scatter criteria, because these cells are larger and more granular than hematopoietic cells. Additionally, staining for CD
Using VMD: An Introductory Tutorial
Sui, H., Han, B.‐G., Lee, J.K., Walian, P., and Jap, B.K. 2001. Structural basis of water‐specific transport through the AQP1 water channel. Nature 414:872‐878. Tajkhorshid
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