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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Locusta migratoria OBP10 protein (OBP10) (I18L,V57I,N131Q)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Locusta migratoria OBP10 protein (OBP10) (I18L,V57I,N131Q)品名简称:
Recombinant Locusta migratoria OBP10 protein (I18L,V57I,N131Q)货号:
CSB-EP4487LHY(M)规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
OBP10Uniprot No.:
H2CSN4别名:
/种属:
Locusta migratoria (Migratory locust)蛋白长度:
Full Length来源:
E.coli分子量:
21.0 kDa表达区域:
1-133aa(I18L,V57I,N131Q)氨基酸序列:
AISESMSRAEEAASKIDLPELFEECNETFTIPKVTLNYFFSHGRLQNENDYGSKCFIHCLTDRSGEIDSDGNFDVDLIKVMTRRFPNETNIEGLNEMVETCVADRGETDFCERAYGLVSCLVKEKLARLGQSH蛋白标签:
N-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
/功能2:
/文献:
"Identification and characterization of novel odorant-binding proteins (OBPs) in locust transcriptomes of two phases." Guo W., Wang X., Kang L. Submitted (JUN-2011)研究领域:
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文献和实验-HCl,pH 7.5,150 mmol/L NaCl,0.1% (V/V) Tween-20。 ( 2 ) 封闭液:2% 牛血清白蛋白(BSA;Sigma, St. Louis, MO) 溶解在 TBST 中。 ( 3 ) [ γ33- P ] ATP,250 μCi/ml ( Amersham Pharmacia Biotech Europe,Freiburg,Germany)。 ( 4 ) CK 2α 缓冲液:25 mmol/L Tris-HCl pH 8.5,10
特异性结合的单克隆抗体1。与Rho1D4抗体结合的表位可以作为针对膜蛋白的超高特异性纯化标记。通过基因修饰可在欲研究的目标(膜)蛋白C端加上Rho1D4标记。一旦加载上该序列,即可用装载有Rho1D4抗体的亲和基质去捕获目标蛋白,随后添加Rho1D4肽,通过竞争性结合基质抗体的方式来洗脱目标蛋白。采用这种方法,相比改变pH值等其他洗脱方式,更加地温和。图1:假设有3个跨膜域的膜蛋白。Rho1D4标签加到膜蛋白C端,它的序列是T-E-T-S-Q-V-A-P-A2) Rho1D4的历史Rho1D4的表位
strep-tag 对 Strep-Tactin XT的亲和力,大规模纯化 CB2 蛋白;然后通过 WB 检测 MBP-CB2 的表达情况,G 蛋白激活实验检测 CB2 的功能活性。 3、CB2 蛋白的纯化的策略:根据不同构建载体表达的情况及 Strep-Tactin 亲和力的测试,作者通过串联亲和层析对 CB2 蛋白进行纯化,分别使用 C 端 His10 和 N 端 twin-Strep-tag。首先使用 Ni-resin 进行第一步纯化后,再从含有 CB2 蛋白的粗提物进行透析,用 TEV 蛋白
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