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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Cat Interleukin-1 (IL1RN)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Cat Interleukin-1 (IL1RN)品名简称:
Recombinant Cat IL1RN protein货号:
CSB-MP3932CA规格:
20μg价格:
1536LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
IL1RNUniprot No.:
A0A337SPR9别名:
/种属:
Felis catus (Cat) (Felis silvestris catus)蛋白长度:
Full Length来源:
Mammalian cell分子量:
48.0 kDa表达区域:
1-182aa氨基酸序列:
MRSWREKCFGNHKQFFQGQQGLPFGGETACHPLGKRPCRMQAFRIWDVNQKTFYLRNNQLVAGYLQGPSTKLEEKLNVVPIESHAMFLGIHGGKLCLACVKSGDETRLQLEAVNITDLSKNKEQDKRFTFIRSDSGPTTSFESAACPGWFLCTALEADRPVSLTNTPEEAMLVTKFYFQMER蛋白标签:
N-terminal hFc-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
18-28 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
/功能2:
/文献:
/研究领域:
Cancer请点击“在线询价”找客服索取产品说明书及引用文献目录,产品实时更新参数、价格信息、更多文献信息可以查看cusabio官网
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文献和实验为细胞培养上清,因该类样本干扰因素 较多,如:细胞状态、细胞数量、采样时间等,所以可能存在检测不出的情况。6. 某些天然蛋白或重组蛋白,包括原核及真核重组蛋白,可能因为与本产品所使用的检测抗体及捕获抗体不匹配,而不被检测出。7. 建议使用新鲜样本,保存时间过长可能会存在蛋白降解或变性导致实验结果偏差。操作步骤1. 加样:分别设标准孔、待测样品孔、空白孔。设标准孔 7 孔,依次加入 100μL 不同浓度的标准品 (见试剂准备 2)。空白孔加 100μL(见试剂准备第二步最后一管),余孔加待测样品
㈠无血清培养基和试剂被广泛的应用于培养哺乳动物和无脊动物细胞以制备单克隆抗体,病毒抗原和重组蛋白等。大多数的无血清产品含有向细胞内转运离子的转铁蛋白和调节葡萄糖摄取量的胰岛素,以及一些蛋白质如清蛋白,纤连蛋白,胎球蛋白等,这些蛋白在细胞培养中发挥各种不同功能,如吸附毒性化合物,抗生物反应器剪切力,提供细胞贴壁所需的基质,作为脂类和其他生长因子的载体等。 ㈡它的优点: 更高的成分限定性 各批产品之间更高的产品质量一致性 简化提纯和下游生产过程 便于控制培养的生理环境
血液 CD66b+ 中性粒细胞(PMN)和胰腺癌组织 CD45+ 免疫细胞 单细胞捕获平台:BD RhapsodyTM 单细胞实验分组: HC-PMN(健康组)×2;CP-PMN(慢性胰腺炎组)×2;PDAC-PMN 细胞×5;PDAC免疫细胞×5 主要技术手段: 单细胞测序,空间转录组测序,转录组测序,蛋白组、代谢组质谱分析,免疫荧光,免疫组化,pcr(qpcr)分析 单细胞分析工具应用: 细胞聚类分析,subCluster 分析,差异基因表达分析,GO/Pathway 分析,拟时序分析
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