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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Protein IL-40 (C17orf99)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100μg/20μg
| 规格: | 1mg | 产品价格: | ¥46572.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥11088.0 |
| 规格: | 20μg | 产品价格: | ¥3960.0 |
纯度:
Greater than 85% as determined by SDS-PAGE.基因名:
C17orf99Uniprot No.:
Q6UX52别名:
Interleukin-40种属:
Homo sapiens (Human)蛋白长度:
Full Length of Mature Protein来源:
Mammalian cell分子量:
56.0 kDa表达区域:
21-265aa氨基酸序列:
REEEITPVVSIAYKVLEVFPKGRWVLITCCAPQPPPPITYSLCGTKNIKVAKKVVKTHEPASFNLNVTLKSSPDLLTYFCWASSTSGAHVDSARLQMHWELWSKPVSELRANFTLQDRGAGPRVEMICQASSGSPPITNSLIGKDGQVHLQQRPCHRQPANFSFLPSQTSDWFWCQAANNANVQHSALTVVPPGGDQKMEDWQGPLESPILALPLYRSTRRLSEEEFGGFRIGNGEVRGRKAAAM蛋白标签:
C-terminal hFc-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
Probable B cell-associated cytokine that plays a role in the regulation of humoral immune responses. Involved in lymphocyte B cell development and immunoglobulin/IgA production.内毒素:
Not test.SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.LC-MS Image Description/Western Blot:
/Product types:
In Stock ProteinBiological_Activity:
/Research Areas:
Cell BiologyReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
"Identification of IL-40, a novel B cell-associated cytokine." Catalan-Dibene J., Vazquez M.I., Luu V.P., Nuccio S.P., Karimzadeh A., Kastenschmidt J.M., Villalta S.A., Ushach I., Pone E.J., Casali P., Raffatellu M., Burkhardt A.M., Hernandez-Ruiz M., Heller G., Hevezi P.A., Zlotnik A. J. Immunol. 199:3326-3335(2017)Function:
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文献和实验Human IL-2 Enzyme Immunoassay (EIA)
Interleukin-2 (IL-2) is a multi-functional protein most notably known for the ability to induce the proliferation and maturation of activated T cells. Additional biological functions include: Stimulus for the division of activated
【原创】Protein A Sepharose 之进化篇。。。
洗脱条件比较剧烈,最后收集的蛋白质很有可能变性,或者是复性困难。 这种洗脱条件剧烈的Protein A 柱结合的重组蛋白质A一般具有5个串联结构域:E、D、A、B、C。这种重组蛋白A虽然除去了不重要的非结合域,仅由5个结构域组成,每个域均可以和IgG的Fc段结合,但不同的域结合强度略有差异。因此洗脱条件不均一,而且经常需要较低的pH值。如果减少串联结构域的个数,并且采取同型结构域串联,就可以避免不同结构域与抗体Fc 段亲和性的差异,洗脱条件会温和而均一。为此,我们可以做一个试验,比较一下
Experimental Applications: Human Acetylcholinesterase as a Model Nervous System Protein
, 5 ng in vitro-transcribed AChE mRNA directed the production of catalytically active recombinant human acetylcholinesterase (rHAChE), at levels of approx 10-fold above background oocyte levels (Table 6 ). Oocyte-produced rHAChE was sensitive to the AChE
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