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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃/-80℃
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Horse Plasminogen activator inhibitor-1 (PAI-1), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100ug/20ug
| 规格: | 1mg | 产品价格: | ¥15792.0 |
|---|---|---|---|
| 规格: | 100ug | 产品价格: | ¥5370.0 |
| 规格: | 20ug | 产品价格: | ¥3324.0 |
Research Areas
CancerUniprot ID
Q8MI31Gene Names
PAI-1Alternative Name(s)
/Lead Time
3-7 business daysOrganism
Equus caballus (Horse)Source
E.coliExpression Region
1-280aaProtein Length
PartialTag Info
Tag-FreeTarget Protein Sequence
GPWNKDEITTADAIFVQRDLKLVKGFMPRFFKLFHTTVKQVDFSEVERARFIINDWVKKYTKGMISDLLDEGAVDELTRLVLVNALYFNGQWKTPFPESGTHHRLFHKSDGSTASVSMMAQTNKFNYAEFTTPDGHYYDILELPYHGDTLSMFIAAPYEKEVPLSALTNILDAQLISQWKRSMTRVVRLLVLPKFSLESEVDLRRPLENLGMTDMFRPNQADFTSISNQELLHVSQALQKVKIEVTESGTVASSSTGFVASARMAPEEIIMDRPFLFVVRMW
32.1 kDaPurity
Greater than 85% as determined by SDS-PAGE.Endotoxin
Not test.Biological_Activity
/Form
Liquid or Lyophilized powderBuffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.Relevance
/Function
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文献和实验CML病人获相对较好的治疗效果。 Hughes等(3)将1106例CML病人随机分为imatinib(蛋白激酶抑制剂)治疗组和INF-a+卡铂治疗组,用FQ-PCR检测2个治疗组病人血液中bcr-abl的转录水平,结果显示imatinib治疗组12个月后bcr-abl的转录水平降低了3个数量级,治疗效果明显优于INF-a+卡铂治疗组。CML病人bcr/abl的转录水平降低了3个数量级或无转录者,预后较好。 4. FQ-PCR在乳腺癌中的应用 Weigelt
的治疗效果。Hughes等(3)将1106例CML病人随机分为imatinib(蛋白激酶抑制剂)治疗组和INF-a+卡铂治疗组,用FQ-PCR检测2个治疗组病人血液中bcr-abl的转录水平,结果显示imatinib治疗组12个月后bcr-abl的转录水平降低了3个数量级,治疗效果明显优于INF-a+卡铂治疗组。CML病人bcr/abl的转录水平降低了3个数量级或无转录者,预后较好。4、FQ-PCR在乳腺癌中的应用 Weigelt等(4)用FQ-PCR方法检测94例乳腺癌转移的病人外周血CK19、p1B、PS
生长因子(EGF)激活的信号转导通路,Fujiwara等[8]在研究Hp毒素及EGF对胃癌KatoⅢ细胞共同作用的过程中发现,不影响细胞活力的VacA毒素剂量可显著抑制EGF与表皮生长因子受体(EGFr)的结合,并减少EGF刺激引起的细胞增殖达22%。Pai等[9]用EGF作用于胃癌KATOⅢ细胞后,可以增强EGFr的表达及其自身磷酸化,VacA可以抑制上述过程。由上可知,VacA干扰了EGF介导的信号转导通路。可能是VacA与RPTPβ的结合促进EGF受体蛋白酪氨酸的去磷酸化,导致EGF作用减弱
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