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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Bat coronavirus HKU9 Nucleoprotein (N)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Bat coronavirus HKU9 Nucleoprotein (N)品名简称:
Recombinant Bat coronavirus HKU9 N protein货号:
CSB-EP385259BOVa0规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
NUniprot No.:
A3EXH0别名:
(Nucleocapsid protein)(NC)(Protein N)种属:
Bat coronavirus HKU9 (BtCoV) (BtCoV/HKU9)蛋白长度:
Full Length来源:
E.coli分子量:
54.2 kDa表达区域:
1-468aa氨基酸序列:
MSGRNRSRSGTPSPKVTFKQESDGSDSESERRNGNRNGARPKNNNSRGSAPKPEKPKAAPPQNVSWFAPLVQTGKAELRFPRGEGVPVSQGVDSTYEHGYWLRTQRSFQKGGKQVLANPRWYFYYTGTGRFGDLRFGTKNPDIVWVGQEGANINRLGDMGTRNPSNDGAIPVQLAGGIPKGFYAEGRGSRGNSRSSSRNSSRASSRGNSRASSRGASPGRPAANPSTEPWMAYLVQKLERLESQVSGTKPATKNPVQVTKNEAAANAKKLRHKRTAHKGSGVTVNYGRRGPGDLEGNFGDREMIKLGTDDPRFAAAAQMAPNVSSFLFMSHLSTRDEDDALWLHYKGAIKLPKDDPNYEQWTKILAENLNAYKDFPPTEPKKDKKKKEETAQDTVIFEDASTGTDQTVVKVWVKDQDAQTDDEWLGGDETVYEDEDDRPKTQRRHKKRGSTASRVTIADPTNAGAERS蛋白标签:
N-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication.功能2:
/文献:
"Comparative analysis of twelve genomes of three novel group 2c and group 2d coronaviruses reveals unique group and subgroup features." Woo P.C.Y., Wang M., Lau S.K.P., Xu H.F., Poon R.W.S., Guo R., Wong B.H.L., Gao K., Tsoi H.-W., Huang Y., Li K.S.M., Lam C.S.F., Chan K.-H., Zheng B.-J., Yuen K.-Y. J. Virol. 81:1574-1585(2007)研究领域:
Microbiology请点击“在线询价”找客服索取产品说明书及引用文献目录,产品实时更新参数、价格信息、更多文献信息可以查看cusabio官网
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文献和实验Overview of Protein Microarrays
., Han, Y., Zhang, Y., Song, Y., Yao, X., Tao, Y., Zeng, H., Yang, H., Wang, J., Zhu, H., Chen, Z.‐N., and Wu, L. 2007. A protein chip approach for high‐throughput antigen identification and characterization. Proteomics 7:2151‐2161
Phenotypic Analysis of BAT versus WAT Differentiation
BAT for UCP1 protein. Left panels show the DAPI nuclear counterstaining; right panels show the Alexa647 signal of the conjugated secondary antibody. Top: IgG isotype control as primary antibody; bottom: rabbit anti UCP1 primary antibody
Strategies to Optimize Protein Expression in E. coli
Peng, L., Xu, Z.N., Fang, X.M., Wang, F., Yang, S., and Cen, P.L. 2004. Preferential codons enhancing the expression level of human beta‐defensin‐2 in recombinant Escherichia coli. Protein Pept. Lett. 11:339
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