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- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Actin, alpha cardiac muscle 1 (ACTC1), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human Actin, alpha cardiac muscle 1 (ACTC1), partial品名简称:
Recombinant Human ACTC1 protein, partial货号:
CSB-BP001221HU规格:
20μg价格:
3168LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
ACTC1Uniprot No.:
P68032别名:
(Alpha-cardiac actin)种属:
Homo sapiens (Human)蛋白长度:
Partial来源:
Baculovirus分子量:
47.4 kDa表达区域:
3-377aa氨基酸序列:
DDEETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDEAGPSIVHRKCF蛋白标签:
C-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.功能2:
/文献:
"ACD toxin-produced actin oligomers poison formin-controlled actin polymerization." Heisler D.B., Kudryashova E., Grinevich D.O., Suarez C., Winkelman J.D., Birukov K.G., Kotha S.R., Parinandi N.L., Vavylonis D., Kovar D.R., Kudryashov D.S. Science 349:535-539(2015)研究领域:
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文献和实验的实验环境和样品的前处理。在某些特殊条件下,一些内参蛋白的表达也不是绝对稳定的,会发生变化,也就是我们常说的,内参不齐。 下面我们来看下不同内参的适用环境: 【Actin】肌动蛋白,是细胞的一种重要骨架蛋白。Actin 大致可分为六种,其中四种是不同肌肉组织特异性的,其余两种广泛分布于各种组织中,包括 β-actin和γ-non-muscle actin。β-actin 广泛分布于细胞浆内,表达量非常丰富,其含量占所有细胞总蛋白的 50%。但是在一些少量特殊的情况下,如脂肪组织,β-actin
different in isometrically contracting cardiac myofibrils from right ventricle of WT and Tg-D166V mice. To avoid averaging over ensembles of molecules composing muscle fibers, the data was collected from a single molecule. Kinetics were derived by tracking
-tubulin。一般我们选择内参与要检测的目的蛋白的分子量最好相差5KD以上。因此你要知道你检测的蛋白的分子量来选择合适的内参! actin即肌动蛋白,是细胞的一种重要骨架蛋白。actin大致可分为六种,其中四种是不同肌肉组织特异性的,包括alpha-skeletal muscle actin、alpha-cardiac muscle actin、alpha-smooth muscle actin和gamma-smooth muscle actin,其余两种广泛分布于各种组织中,包括beta-actin(β
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