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- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Dog Interleukin-13 (IL13)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Dog Interleukin-13 (IL13)品名简称:
Recombinant Dog IL13 protein货号:
CSB-EP868226DO规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
IL13Uniprot No.:
Q9N0W9别名:
(IL-13)种属:
Canis lupus familiaris (Dog) (Canis familiaris)蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
16.5 kDa表达区域:
19-131aa氨基酸序列:
SPSPVTPSPTLKELIEELVNITQNQASLCNGSMVWSVNLTAGMYCAALESLINVSDCSAIQRTQRMLKALCSQKPAAGQISSERSRDTKIEVIQLVKNLLTYVRGVYRHGNFR蛋白标签:
N-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Cytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Positively regulates IL31RA expression in macrophages.功能2:
/文献:
"Canine interleukin-13: molecular cloning of full-length cDNA and expression of biologically active recombinant protein." Yang S., Boroughs K.L., McDermott M.J. J. Interferon Cytokine Res. 20:779-785(2000)研究领域:
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文献和实验做哮喘动物模型这些年,AAV-IL13是我见过最高效便捷的一种
酸性粒细胞(Eosinophils)和中性粒细胞(Neutrophils)的增加最为剧烈,淋巴细胞也有一定程度增加;同时,嗜酸性粒细胞关键趋化因子Ccl11(Eotaxin-1)在肺部呈现剂量依赖性上调。 杯状细胞化生与黏液分泌增加 肺组织PAS染色(糖原染色)显示,AAV-IL13处理的小鼠气道上皮发生强烈的杯状细胞化生(Goblet cell metaplasia)和黏液过度分泌,高剂量下甚至引发严重的黏液栓塞;粘蛋白基因Muc5ac的表达量显著上调。 2、群转录组特征
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【讨论】Innate immunity: 'Natural helper' cells identified
lineage (LIN) markers but express interleukin-7 receptor-α (IL-7Rα) and ST2 (a subunit of IL-33R), along with several other cell surface molecules. Further analysis showed that these cells are of lymphoid lineage but are distinct from B and T cells
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