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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃/-80℃
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Haemophilus influenzae Peptidoglycan-associated lipoprotein (pal), Biotinylated
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100ug/20ug
| 规格: | 1mg | 产品价格: | ¥15792.0 |
|---|---|---|---|
| 规格: | 100ug | 产品价格: | ¥5370.0 |
| 规格: | 20ug | 产品价格: | ¥3324.0 |
Research Areas
OthersUniprot ID
P10324Gene Names
palAlternative Name(s)
(PAL)(15 kDa peptidoglycan-associated lipoprotein)(PC protein)(Outer membrane protein P6)(OMP P6)Lead Time
13-23 business daysOrganism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)Source
E.coliExpression Region
20-153aaProtein Length
Full Length of Mature ProteinTag Info
N-terminal MBP-tagged and C-terminal 6xHis-Avi-taggedTarget Protein Sequence
CSSSNNDAAGNGAAQTFGGYSVADLQQRYNTVYFGFDKYDITGEYVQILDAHAAYLNATPAAKVLVEGNTDERGTPEYNIALGQRRADAVKGYLAGKGVDAGKLGTVSYGEEKPAVLGHDEAAYSKNRRAVLAYMW
62.0 kDaPurity
Greater than 85% as determined by SDS-PAGE.Endotoxin
Not test.Biological_Activity
/Form
Liquid or Lyophilized powderBuffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.Relevance
Part of the Tol-Pal system, which plays a role in outer membrane invagination during cell division and is important for maintaining outer membrane integrity.Function
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文献和实验RIP技术(RNA结合蛋白免疫沉淀),研究肿瘤发生和转移中microRNA失调的有力工具
, RISC),然后通过不完全的碱基互补靶向到目标mRNA的3’UTR区,再通过引导酶切影响了mRNA的稳定性,或直接阻碍翻译过程,从而介导特异mRNA靶标的转录后基因沉默。 miRNA参与转录后调控网络。miRNA靶向到目标mRNA的3’UTR区,抑制靶mRNA的蛋白表达。(摘自Chatterjee & Pal, Biol Cell. 2009) miRNA对许多生理过程产生重要影响,如细胞生长、凋亡、应激应答、干细胞分化潜能的维持
,这些小分子RNA 存在于几乎所有较高等的真核生物细胞中,对生物体具有非常重要的调控功能。它们通过各种序列特异性的RNA 基因沉默作用,包括RNA 干扰 (RNAi)、翻译抑制、异染色质形成等,调控诸如生长发育、应激反应、沉默转座子等各种各样的细胞进程。随着对这些小分子调控RNA 的发现,一些RNase III 酶家族成员、Argonaute 蛋白质家族成员及RNA 结合蛋白质等先后被鉴定为小RNA 的胞内蛋白质合作者,参与小RNA 的加工成熟和在细胞内行使功能。本综述简介一些RNA 沉默作用途径中
自身的DNA,分解外来的DNA,以保护和维持自身遗传信息的稳定,这对细菌的生存和繁衍具有重要意义。这就是限制性核酸内切酶名称中“限制”二字概念的由来。 二、限制性核酸内切酶的命名 按酶的来源的属、种名而定,取属名的第一个字母与种名的头两个字母组成的三个斜体字母作略语表示;如有株名,再加上一个字母,其后再按发现的先后写上罗马数字。例如:从流感嗜血杆菌d株(Haemophilus influenzae d)中先后分离到3种限制酶,则分别命名为HindⅠ、HindⅡ和HindⅢ。 三
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