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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Ceruloplasmin (CP), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100μg/20μg
| 规格: | 1mg | 产品价格: | ¥14796.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥3468.0 |
| 规格: | 20μg | 产品价格: | ¥1836.0 |
纯度:
Greater than 85% as determined by SDS-PAGE.基因名:
CPUniprot No.:
P00450别名:
(Ferroxidase)种属:
Homo sapiens (Human)蛋白长度:
Partial来源:
E.coli分子量:
32.1 kDa表达区域:
807-1050aa氨基酸序列:
ILGPQLHADVGDKVKIIFKNMATRPYSIHAHGVQTESSTVTPTLPGETLTYVWKIPERSGAGTEDSACIPWAYYSTVDQVKDLYSGLIGPLIVCRRPYLKVFNPRRKLEFALLFLVFDENESWYLDDNIKTYSDHPEKVNKDDEEFIESNKMHAINGRMFGNLQGLTMHVGDEVNWYLMGMGNEIDLHTVHFHGHSFQYKHRGVYSSDVFDIFPGTYQTLEMFPRTPGIWLLHCHVTDHIHAGM蛋白标签:
N-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
Ceruloplasmin is a blue, copper-binding (6-7 atoms per molecule) glycoprotein. It has ferroxidase activity oxidizing Fe(2+) to Fe(3+) without releasing radical oxygen species. It is involved in iron transport across the cell membrane. Provides Cu(2+) ions for the ascorbate-mediated deaminase degradation of the heparan sulfate chains of GPC1. May also play a role in fetal lung development or pulmonary antioxidant defense.内毒素:
Not test.SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.LC-MS Image Description/Western Blot:
/Product types:
Developed ProteinBiological_Activity:
/Research Areas:
Signal TransductionReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
"Single-chain structure of human ceruloplasmin: the complete amino acid sequence of the whole molecule." Takahashi N., Ortel T.L., Putnam F.W. Proc. Natl. Acad. Sci. U.S.A. 81:390-394(1984)Function:
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文献和实验X-100或脱氧胆酸钠)和低浓度变性剂(2mol/L尿素或盐酸胍等)洗涤除去脂类和膜蛋白,这一步很重要,否则会导致包涵体溶解和复性的过程中重组蛋白质的降解[6、7、8]。 包涵体的溶解必须用很强的变性剂,如8mol/L尿素、6~8mol/L盐酸胍,通过离子间的相互作用破坏包涵体蛋白间的氢键而增溶蛋白。其中尿素的增溶效果稍差,异氰盐酸胍最强;去污剂,如SDS[7],可以破坏蛋白内的疏水键,可以增溶几乎所有的蛋白,但由于无法彻底去除而不允许用在制药行业中;酸,如70%甲酸[9],可以破坏蛋白
案例详情 患者男,65 岁,于 2020 年 11 月 22 日来我院体检。 心电图检查:1、窦性心律,2、正常心电图。实验室检查:肝功能及肾功能正常,血糖 6.23mmoL/L,甘油三酯 2.75mmoL/L,胆固醇及高密度脂蛋白胆固醇正常,乙肝五项都阴性,血常规及尿常规都正常。所查的两项肿瘤标志物中唯独甲胎蛋白异常升高。 按照常理,这么高的甲胎蛋白应差不多是肝癌了吧! 进一步诊查 然而,该体检者的肝脏超声结果却未发现有占位性病变。 1. 彩色多普勒超声 (泌尿系统、肝胆脾 + 双肾、甲状
/bodyfluid/ 简介:人体体液蛋白组研究数据库。提供人体各种体液的蛋白质组数据,包括血浆/血清、尿液、乳汁、泪、汗液、唾液、骨髓液、脑脊液、胃液等。 5.BloodExpress 网址:http://hscl.cimr.cam.ac.uk/bloodexpress/ 简介:小鼠造血过程基因表达数据库 6.CentrosomeDB(human centrosomal proteins database) 网址:http://centrosome.dacya.ucm.es 简介
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