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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃/-80℃
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Complement component C9 (C9), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100ug/20ug
| 规格: | 1mg | 产品价格: | ¥14796.0 |
|---|---|---|---|
| 规格: | 100ug | 产品价格: | ¥3468.0 |
| 规格: | 20ug | 产品价格: | ¥1836.0 |
Research Areas
ImmunologyUniprot ID
P02748Gene Names
C9Alternative Name(s)
/Lead Time
13-23 business daysOrganism
Homo sapiens (Human)Source
E.coliExpression Region
74-155aaProtein Length
PartialTag Info
N-terminal 6xHis-GST-taggedTarget Protein Sequence
NGKRCTDAVGDRRQCVPTEPCEDAEDDCGNDFQCSTGRCIKMRLRCNGDNDCGDFSDEDDCESEPRPPCRDRVVEESELARTMW
40.7 kDaPurity
Greater than 90% as determined by SDS-PAGE.Endotoxin
Not test.Biological_Activity
/Form
Liquid or Lyophilized powderBuffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.Relevance
Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C9 is the pore-forming subunit of the MAC.Function
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文献和实验,于室温处理 1 小时进行终止。+4°C 环境下,将神经元细胞与神经元标志微管相关蛋白 2 (microtubule-associated protein 2,MAP-2) (英国剑桥Abcam,ab11267, HM-2,1:600 稀释)小鼠单克隆抗体共同孵育,孵育过夜。第 2 天,将细胞与山羊抗小鼠二抗(Alexa Fluor® 488 goat anti-mouse IgG (H+L) (Invitrogen,1:250 稀释)共同孵育。使用DMI6000 B 倒置显微镜(德国,Leica
sapiens 3 BAC RP11-144C9 (R... 30 6.7 gi|22549584|gb|AC131205.3| Homo sapiens 12 BAC RP11-185H22 ... 30 6.7 gi|11465016|gb|AC078854.16| Homo sapiens 3q BAC RP11-480B17... 30 6.7 gi|29569255|gb|AC138940.3| Homo sapiens chromosome 5 clone ... 30 6.7 gi
酶),所以C3及其后续成分不被活化。因此本病不像C3~C8缺陷那样容易发生感染。 大部分CIINH缺陷病人与遗传有关,另有约15%的病人无遗传史,其CIINH虽有抗原性但无活性(部分可产生正常CIINH,并非完全缺陷)。前者称为I型血管性水肿,后者称为Ⅱ型血管性水肿(Alsenz等,1987)。 血管性水肿可用提纯的CIINH治疗,据称有效,亦可给以男性激素制剂以促进肝合成CIINH,预防水肿的发生。 C4结合蛋白:C4结合蛋白(C4 binding protein, C4
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