- ￥2680
- Sino Biological
- 北京
- GMP-10602-HNAE-L
- 2025年08月12日
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- 详细信息
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human TNF-alpha / TNFA Protein
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
100 µg
重组人 TNF-alpha / TNFA 蛋白（产品说明）
蛋白名称：Recombinant Human TNF-alpha / TNFA Protein
蛋白构建：A DNA sequence encoding the human TNF-α soluble form (NP_000585.2) (Val77-Leu233) was expressed with an initial Met at the N-terminus.
表达宿主：E. coli
蛋白纯度：≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
蛋白活性：Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 3-30 pg/mL. The specific activity of recombinant human TNF-alpha is >36,000IU/μg, which is calibrated against the human TNF-alpha WHO International Standard (NIBSC code: 12/154).
蛋白内毒素：< 5 EU per mg of the protein.
预测N端：Met
蛋白分子量：The recombinant human TNF-α consists of 158 amino acids and has a predicted molecular mass of 17.5 kDa.
蛋白NP号：NP_000585.2
蛋白氨基酸序列：Val77-Leu233
蛋白标签：Native
蛋白保存条件：-20℃ to -80℃
蛋白名称：Recombinant Human TNF-alpha / TNFA Protein
蛋白构建：A DNA sequence encoding the human TNF-α soluble form (NP_000585.2) (Val77-Leu233) was expressed with an initial Met at the N-terminus.
表达宿主：E. coli
蛋白纯度：≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
蛋白活性：Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 3-30 pg/mL. The specific activity of recombinant human TNF-alpha is >36,000IU/μg, which is calibrated against the human TNF-alpha WHO International Standard (NIBSC code: 12/154).
蛋白内毒素：< 5 EU per mg of the protein.
预测N端：Met
蛋白分子量：The recombinant human TNF-α consists of 158 amino acids and has a predicted molecular mass of 17.5 kDa.
蛋白NP号：NP_000585.2
蛋白氨基酸序列：Val77-Leu233
蛋白标签：Native
蛋白保存条件：-20℃ to -80℃
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重组人 TNF-alpha / TNFA 蛋白
￥2680
