重组人 TNF-alpha / TNFA 蛋白(产品说明)
蛋白名称:Recombinant Human TNF-alpha / TNFA Protein
蛋白构建:A DNA sequence encoding the human TNF-α soluble form (NP_000585.2) (Val77-Leu233) was expressed with an initial Met at the N-terminus.
表达宿主:E. coli
蛋白纯度:≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
蛋白活性:Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 3-30 pg/mL. The specific activity of recombinant human TNF-alpha is >36,000IU/μg, which is calibrated against the human TNF-alpha WHO International Standard (NIBSC code: 12/154).
蛋白内毒素:< 5 EU per mg of the protein.
预测N端:Met
蛋白分子量:The recombinant human TNF-α consists of 158 amino acids and has a predicted molecular mass of 17.5 kDa.
蛋白NP号:NP_000585.2
蛋白氨基酸序列:Val77-Leu233
蛋白标签:Native
蛋白保存条件:-20℃ to -80℃