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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
12 months
- 英文名:
Recombinant Rubella virus/RUBV Polyprotein Protein, N-His
- 供应商:
武汉益普生物科技有限公司
- 规格:
50μg/100μg/1mg
| 规格: | 50μg | 产品价格: | ¥1280.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥1880.0 |
| 规格: | 1mg | 产品价格: | ¥9776.0 |
| 产品名 | Recombinant Rubella virus/RUBV Polyprotein Protein, N-His |
| 货号 | YVV22801 |
| 别名 | Polyprotein |
| 表达系统 | E. coli |
| Accession号 | Q8QL55 |
| 种属 | Rubella virus (RUBV) |
| 蛋白长度 | Asn127-Arg277 |
| 预测分子量 | 19.32 kDa |
| 性质 | Recombinant |
| 内毒素水平 | Please contact with the lab for this information. |
| 纯度 | >90% as determined by SDS-PAGE. |
| 应用 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 状态 | Lyophilized |
| 保存溶液 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 运输 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 稳定性和存储 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 说明 | For research use only. |

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文献和实验Genetic Engineering and Chemical Conjugation of Potato Virus X
Here we report the genetic engineering and chemical modification of potato virus X (PVX) for the presentation of various peptides, proteins, and fluorescent dyes, or other chemical modifiers. Three different ways of genetic engineering
Recombinant DNA Engineering, Or Cloning Genes In Plasmids
and thus fusion proteins. For example there might be sequence for a fluorescent protein such as GFP or a peptide tag such as HA upstream of MCS (and downstream of promoter) so that an insert in frame will lead to the generation of a fusion protein, an N-terminal
, and usually final protein yield between 0.1 and 2 mg/g cells is acceptable. Another concern is protein degradation. Especially with Pichia , protease activity during cell lysis and purification is always an issue. The importance of N-terminal degradation
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