- ￥1280 - 9776
- AntibodySystem
- France
- YVV10001
- 2025年12月06日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
12 months
- 英文名：
Recombinant ASFV I267L Protein, N-GST
- 供应商：
武汉益普生物科技有限公司
- 规格：
50μg/100μg/1mg
| 规格： | 50μg | 产品价格： | ￥1280.0 |
|---|---|---|---|
| 规格： | 100μg | 产品价格： | ￥1880.0 |
| 规格： | 1mg | 产品价格： | ￥9776.0 |
| 产品名 | Recombinant ASFV I267L Protein, N-GST |
| 货号 | YVV10001 |
| 别名 | I267L CDS, I267L, I267L CDS protein |
| 表达系统 | E. coli |
| Accession号 | A0A6V6ZED8 |
| 种属 | African swine fever virus |
| 蛋白长度 | Met21-Asn243 |
| 预测分子量 | 52.47 kDa |
| 性质 | Recombinant |
| 内毒素水平 | Please contact with the lab for this information. |
| 纯度 | >90% as determined by SDS-PAGE. |
| 应用 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 状态 | Lyophilized |
| 保存溶液 | Lyophilized from a solution in PBS pH 7.4, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 运输 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 稳定性和存储 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 说明 | For research use only. |
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文献和实验Purification of recombinant sBRF M166L
culture with 0.4 mM IPTG (4 ml of 0.1 M IPTG per 1000 ml) and grow at 30 deg. for four hours. Take a small sample of the induced and uninduced cultures and save for analysis of whole cell protein by SDS PAGE. Harvest cells by centrifugation (4-6g
Purification of recombinant sBRF M166L
with 0.4 mM IPTG (4 ml of 0.1 M IPTG per 1000 ml)and grow at 30 deg.for four hours.Take a small sample of the induced and uninduced cultures and save for analysis of whole cell protein by SDS PAGE. Harvest cells by centrifugation (4-6g).Resuspend the cell
Purification of Antibody Light Chains by Metal Affinity and Protein L Chromatography
(five or six His residues) placed at either the C- or N-terminus of a recombinant protein can form a stable chelate with immobilized transition metals. This allows fractionation of the target protein to 90–95% purity levels in a single chromatographic step (2 –4 ). Metals
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