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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Carrier-protein conjugated synthetic peptide surrounding phospho Ser376 of human IKK gamma. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX134550
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, IHC-P
- 浓度:
1.68 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
IKK gamma (phospho Ser376)
- 抗体英文名:
IKK gamma (phospho Ser376) antibody
- 抗体名:
IKK gamma (phospho Ser376) 抗体
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
IKK gamma (phospho Ser376) antibody detects IKK gamma (phospho Ser376) protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
IKK gamma (phospho Ser376) stained by IKK gamma (phospho Ser376) antibody (GTX134550) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IKK gamma (phospho Ser376) antibody (GTX134550) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献和实验Irradiating Cells - Once cells are confluent tighten the lid of the flasks and take up to the gamma source in the animal house on the third floor (PMCI), if you have a number of flasks you can trypsinise all the flasks and place
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
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