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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Pooled antisera from goats hyperimmunized with human IgM
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
- 克隆性:
Polyclonal
- 标记物:
Purified by antigen-affinity chromatography
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Batch dependent (Please refer to the vial label for the specific concentration.)
- 目录编号:
GTX04120-03
- 级别:
Secondary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Goat
- 应用范围:
WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA, Activation, Depletion, ELISPOT, Multiplexing, Purification
- 浓度:
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点:
Goat Anti-Human IgM (Mu chain)
- 抗体英文名:
Goat Anti-Human IgM (Mu chain) antibody, pre-adsorbed (AP)
- 抗体名:
Goat Anti-Human IgM (Mu chain) 抗体, pre-adsorbed (AP)
- 规格:
1 ml
ELISA analysis of purified human immunoglobulins using serially diluted GTX04120-03 Goat Anti-Human IgM (Mu chain) antibody, pre-adsorbed (AP).
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文献和实验4-Hydroxy-2-Nonenal (4-HNE) Staining by Anti-HNE Antibody
using an anti-4-HNE monoclonal antibody (MAb) (6 –8 ) and labeled goat anti-mouse IgG antibody (9 ).
which employs goat anti-Rabbit HRP.12. Wash filter 3 times 7 minutes with 0.5% BSA in TBST.13. Perform Alkaline phosphatase reaction using NBT and BCIP substrates. I find that CREB usually comes up within several minutes of adding the AP substrates
IMMUNOSCREENING OF WITH ANTIBODY PROBES
. Incubate these membrane sections with secondary antiserum for 1 hour at room temperature (For detecting human antibodies, we use Sigma #A-3150: goat anti-human IgG alkaline phosphatase conjugate, diluted 1:1000) Wash membranes in TBST for 5 minutes
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