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- 2025年12月05日
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货期：1-2天
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MedChemExpress LLC
- 规格：
250 U
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Sge I
MCE 国际站：Sge I
Sge I 限制性内切酶可识别与切割单链或双链 DNA 上含有 5-甲基胞嘧啶的 DNA 靶标。
Description & Advantages:
• Sge I restriction enzyme recognizes and cleaves DNA targets containing 5-methylcytosine on one or both DNA strands, and cuts best at 37℃. To ensure consistent performance, this product contains premixed BSA, which enhances the stability of Sge I and binds contaminants that may be present in DNA preparations.
• Cleavage site:
5’…m5 C N N G (N)9▼…3’
3’… G N N C (N)13▲…5’
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文献和实验Parameters Affecting Capillary Electrophoretic Separation of DNA
Since its introduction in 1930 by Tiselius (1 ), slab-gel electrophoresis (SGE) has been utilized as an important separation technique in molecular biology for the resolution of DNA fragments, polymerase chain reaction (PCR) products
16 DNA Analysis Under Highly Denaturing Conditions in Bare Fused Silica Capillaries
on their size and/or conformation differences. Various denaturing techniques have been developed for both slab gel (SGE) and capillary electrophoresis (CE) formats. The highest possible denaturing ability of the background electrolytes (BGE) of both the sample
下降，说明你的溶剂或样品含有颗粒杂质，正是这些杂质将筛板堵塞引起压力上升。若柱压还高，请与厂商联系。一般情况下，在进样器与保护柱之间接一个在线过滤器便可避免柱压过高的问题，SGE提供的Rheodyne 7315型过滤器就是解决这一问题的最佳选择。
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